Mouse Leptin Matched Antibody Pair Kit (ab214475)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 62.5 pg/ml - 4000 pg/ml
Overview
-
Product name
Mouse Leptin Matched Antibody Pair Kit
See all Leptin kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
26.46 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse Leptin Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse Leptin.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab199082. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
-
Tested applications
Suitable for: ELISA, IAmore details -
Platform
Reagents
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse Leptin Capture Antibody 2 x 50µg 1 x 50µg Mouse Leptin Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse Leptin Lyophilized Protein 2 vials 1 vial -
Research areas
-
Function
May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. -
Involvement in disease
Defects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. -
Sequence similarities
Belongs to the leptin family. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- FLJ94114
- LEP
- LEP_HUMAN
see all -
Database links
- Entrez Gene: 16846 Mouse
- SwissProt: P41160 Mouse
- Unigene: 277072 Mouse
Images
-
Mouse Leptin standard curve.
Standard calibration curve. Background subtracted values are graphed.
