Mouse Leptin ELISA Kit (ab100718)
Key features and details
- Sensitivity: 4 pg/ml
- Range: 4.1 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 120X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Mouse Leptin (lyophilized) 2 vials Leptin Microplate (12 strips x 8 wells) 1 unit Recombinant Mouse Leptin Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. -
Involvement in disease
Defects in LEP may be a cause of obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. -
Sequence similarities
Belongs to the leptin family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- FLJ94114
- LEP
- LEP_HUMAN
see all -
Database links
- Entrez Gene: 16846 Mouse
- SwissProt: P41160 Mouse
- Unigene: 277072 Mouse
Images
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ab100718 Leptin Mouse ELISA Kit
Ms Leptin measured in biological fluids showing quantity (pg) per mL of tested sample
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ab100718 Leptin Mouse ELISA KitMs Leptin measured in cell lysates showing quantity (pg) per mg protein
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Typical Standard CurveRepresentative Standard Curve using ab100718
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Typical Standard CurveRepresentative Standard Curve using ab100718
