Human OLFM4 ELISA Kit (ab267805)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 315.7 pg/ml
- Range: 1171.88 pg/ml - 75000 pg/ml
- Sample type: Cell culture media, Cit plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human OLFM4 ELISA Kit
See all OLFM4 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3.7% Inter-assay Sample n Mean SD CV% Serum 3 3.4% -
Sample type
Serum, Tissue Extracts, Cell culture media, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
315.7 pg/ml -
Range
1171.88 pg/ml - 75000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 86 84% - 87% Tissue Extracts 101 89% - 110% Cell culture media 95 94% - 97% Cit plasma 86 83% - 88% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human OLFM4 ELISA Kit (ab267805) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of OLFM4 protein in serum, cit plasma, tissue extracts, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human OLFM4 with 315.7 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
Notes
Olfactomedin-4 (OLFM4), formerly known as GW112 or hGC-1, is a member of the olfactomedin gene family and predominantly expressed in bone marrow, small intestine, colon, and prostrate. Studies have revealed OLFM4 to play important roles in several cellular functions such as neurogenesis, cell adhesion, cell-cycle regulation, and tumorigenesis. Moreover, studies have suggested OLFM4 to serve as a robust biomarker in studying stem cells and cancer in small intestines, stomach, and colon.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human OLFM4 Capture Antibody 1 x 600µl 10X Human OLFM4 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human OLFM4 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
-
Function
An antiapoptotic factor that promotes tumor growth. Promotes proliferation of pancreatic cancer cells by favoring the transition from the S to G2/M phase. Facilitates cell adhesion. -
Tissue specificity
Expressed during myeloid lineage development. Strongly expressed in the prostate, small intestine and colon and moderately expressed in the bone marrow and stomach. Highly expressed in pancreatic cancer tissues and shows an elevated expression level during the early S phase of the cell cycle. Also expressed at high levels in stomach cancer and colon cancer tissues. -
Sequence similarities
Contains 1 olfactomedin-like domain. -
Domain
The olfactomedin-like domain is involved in the interaction with cadherin. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Secreted > extracellular space. According to PubMed:15059901, found in mitochondrion and nucleus. - Information by UniProt
-
Alternative names
- Antiapoptotic protein GW112
- bA209J19.1
- G-CSF-stimulated clone 1 protein
see all -
Database links
- Entrez Gene: 10562 Human
- Omim: 614061 Human
- SwissProt: Q6UX06 Human
- Unigene: 508113 Human
Images
-
SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
Example of human OLFM4 standard curve in Sample Diluent NS.The OLFM4 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
-
Interpolated concentrations of native OLFM4 in human serum and plasma samples.The concentrations of OLFM4 were measured in duplicates, interpolated from the OLFM4 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100% and plasma (citrate) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean OLFM4 concentration was determined to be 4,710 pg/mL in serum and 4,029 pg/mL in plasma (citrate).
-
Interpolated concentrations of native OLFM4 in human colon tissue extract based on a 400 µg/mL extract load.The concentrations of OLFM4 were measured in duplicate and interpolated from the OLFM4 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean OLFM4 concentration was determined to be 48,973 pg/mL in colon tissue extract.
-
Sandwich ELISA - Human OLFM4 ELISA Kit (ab267805)To learn more about the advantages of recombinant antibodies see here.
