Mouse CCL6 ELISA Kit (ab193719)
Key features and details
- Sensitivity: 18 pg/ml
- Range: 18 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse CCL6 ELISA Kit
See all CCL6 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
18 pg/ml -
Range
18 pg/ml - 1000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 104.2 97% - 113% Plasma 88.31 81% - 96% Cell culture media 110.2 92% - 122% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Mouse -
Product overview
Abcam’s CCL6 mouse ELISA Kit (ab193719) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse CCL6 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for mouse CCL6 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures CCL6 present in the samples. The wells are washed and biotinylated anti-mouse CCL6 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of CCL6 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Detection Antibody CCL6 (biotinylated anti-mouse CCL6) 2 vials Mouse CCL-6 Standards (lyophilized) 2 vials Pre-coated mouse CCL-6 Microplate (12 strips x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Function
CCL6(22-95) and CCL6(23-95) are potent chemoattractants. -
Tissue specificity
Expressed in myelopoietic bone marrow cultures stimulated by GM-CSF. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C-C motif chemokine 6
- c10
- CC chemokine C10
see all -
Database links
- Entrez Gene: 20305 Mouse
- SwissProt: P27784 Mouse
- Unigene: 137 Mouse
Images
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Standard Curve.
Typical Standard Curve in Assay Diluent A using ab193719 CCL6 Mouse ELISA Kit.
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Standard Curve.Typical Standard Curve in Assay Diluent B using ab193719 CCL6 Mouse ELISA Kit.