Mouse Fractalkine Matched Antibody Pair Kit (ab215397)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 250 pg/ml - 16000 pg/ml
Overview
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Product name
Mouse Fractalkine Matched Antibody Pair Kit
See all CX3CL1 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
151.12 pg/ml -
Range
250 pg/ml - 16000 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse Fractalkine Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse Fractalkine.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab206981. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: IA, ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse Fractalkine Capture Antibody 2 x 50µg 1 x 50µg Mouse Fractalkine Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse Fractalkine Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1. -
Tissue specificity
Small intestine, colon, testis, prostate, heart, brain, lung, skeletal muscle, kidney and pancreas. -
Sequence similarities
Belongs to the intercrine delta family. -
Post-translational
modificationsA soluble short 95 kDa form may be released by proteolytic cleavage from the long membrane-anchored form.
O-glycosylated with core 1 or possibly core 8 glycans. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Alternative names
- A 152E5.2
- AB030188
- ABCD 3
see all -
Database links
- Entrez Gene: 20312 Mouse
- SwissProt: O35188 Mouse
- Unigene: 103711 Mouse
Images
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Mouse Fractalkine standard curve.
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Mouse Fractalkine Matched Antibody Pair Kit (ab215397)To learn more about the advantages of recombinant antibodies see here.
