Isopropanol Dehydrogenase Activity Assay Kit (Colorimetric) (ab273418)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate
Overview
-
Product name
Isopropanol Dehydrogenase Activity Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Assay type
Enzyme activity (quantitative) -
Assay duration
Multiple steps standard assay -
Product overview
Isopropanol Dehydrogenase Activity Assay Kit (Colorimetric) (ab273418) provides a convenient tool for sensitive detection of the Isopropanol Dehydrogenase (ADH1-NADP) in a variety of samples (bacterial, yeast or protozoan, animal tissues and cells). In this assay, Isopropanol gets converted to Acetone and NADPH in the presence of NADP+. This results in the development of color in the presence of the developer. This color is proportional to the ADH1-NADP levels in the sample and can be measured at λ = 450 nm.
This assay detects ADH1-NADP activity as low as 0.001 mU in samples.
-
Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests ADH1-NADP Assay Buffer 1 x 25ml ADH1-NADP Developer (Lyophilized) 1 vial ADH1-NADP Positive Control 1 x 100µl ADH1-NADP Substrate 1 x 1ml NADPH Standard (Lyophilized) 1 vial
Images
-
Example data
Example data.
NADPH standard curve.
-
Example dataExample data.
ADH1-NADP Positive Controls (µl/assay).
-
Example dataExample data.
~5 mg of bovine liver, yeast and E.coli cell pellet were homogenized as described in the kit protocol. Serial dilutions were tested to ensure the readings were within the linear range of the Standard Curve. ADH1-NADP activities (mU/mg) of samples were measured and calculated as in the kit protocol.
