Anti-Cytochrome P450 2B6/CYP2B6 antibody (ab223511)
Key features and details
- Goat polyclonal to Cytochrome P450 2B6/CYP2B6
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Cytochrome P450 2B6/CYP2B6 antibody
See all Cytochrome P450 2B6/CYP2B6 primary antibodies -
Description
Goat polyclonal to Cytochrome P450 2B6/CYP2B6 -
Host species
Goat -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human Cytochrome P450 2B6/CYP2B6 aa 200-300 (internal sequence) (Cysteine residue). NP_000758.1.
Database link: P20813 -
Positive control
- WB: HepG2 and K562 cell lysates.
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General notes
This product was previously labelled as Cytochrome P450 2B6
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab223511 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Cytochrome P450 2B6/CYP2B6 antibody (ab223511)All lanes : Anti-Cytochrome P450 2B6/CYP2B6 antibody (ab223511) at 0.1 µg/ml
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate in RIPA buffer
Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) cell lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 56.3 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour.
