Anti-MPP8 antibody (ab124499)
Key features and details
- Rabbit polyclonal to MPP8
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MPP8 antibody
See all MPP8 primary antibodies -
Description
Rabbit polyclonal to MPP8 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human MPP8 aa 400-500 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q99549 -
Positive control
- This antibody gave a positive signal in MCF7 Nuclear and HEK293 whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124499 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications HorseCowChimpanzeeMacaque monkeyGorillaOrangutanApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 97 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 125 kDa (predicted molecular weight: 97 kDa).Target
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Sequence similarities
Contains 4 ANK repeats.
Contains 1 chromo domain. -
Post-translational
modificationsPhosphorylated in M (mitotic) phase. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 467217 Chimpanzee
- Entrez Gene: 54737 Human
- Omim: 611626 Human
- SwissProt: Q99549 Human
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Alternative names
- HSMPP8 antibody
- M-phase phosphoprotein 8 antibody
- M-phase phosphoprotein, mpp antibody
see all
Images
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Western blot - Anti-MPP8 antibody (ab124499)All lanes : Anti-MPP8 antibody (ab124499) at 1 µg/ml
Lane 1 :MCF7 nuclear extract lysate (ab14860)
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?
Additional bands at: 80 kDa (possible non-specific binding)
Exposure time: 2 minutesThe predicted molecular weight of MPP8 is 97 kDa (SwissProt), however we expect to observe a banding pattern around 125 kDa.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab124499 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406
Datasheets and documents
References (0)
ab124499 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-MPP8 antibody (ab124499)All lanes : Anti-MPP8 antibody (ab124499) at 1 µg/ml
Lane 1 :MCF7 nuclear extract lysate (ab14860)
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 97 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?
Additional bands at: 80 kDa (possible non-specific binding)
Exposure time: 2 minutesThe predicted molecular weight of MPP8 is 97 kDa (SwissProt), however we expect to observe a banding pattern around 125 kDa.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab124499 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406
