Anti-MPP8 antibody [EPR19856] (ab214812)
![Anti-MPP8 antibody [EPR19856] (ab214812)](https://www.abcam.com/ps/products/214/ab214812/Images/ab214812-288236-anti-mpp8-antibody-epr19856-western-blot.jpg "Anti-MPP8 antibody [EPR19856] (ab214812)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19856] to MPP8
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse
Overview
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Product name
Anti-MPP8 antibody [EPR19856]
See all MPP8 primary antibodies -
Description
Rabbit monoclonal [EPR19856] to MPP8 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Wild-type mouse embryonic stem (ES) cell lysate; RAW264.7 and L-929 whole cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19856 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MPP8 antibody [EPR19856] (ab214812)All lanes : Anti-MPP8 antibody [EPR19856] (ab214812) at 1/1000 dilution
Lane 1 : Wild-type mouse embryonic stem (ES) cell lysate
Lane 2 : MPP8 knockout mouse embryonic stem (ES) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 97 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsThe cell lysates were kindly provided by our collaborator Dr. Jinzhong Qin, Nanjing University MARC.
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![Western blot - Anti-MPP8 antibody [EPR19856] (ab214812)](https://www.abcam.com/ps/products/214/ab214812/Images/ab214812-288235-anti-mpp8-antibody-epr19856-western-blot.jpg)
Western blot - Anti-MPP8 antibody [EPR19856] (ab214812)All lanes : Anti-MPP8 antibody [EPR19856] (ab214812) at 1/1000 dilution
Lane 1 : RAW264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 2 : L-929 (mouse connective tissue fibroblast cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 97 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?Exposure time : Lane 1: 3 minutes; Lane 2: 15 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Anti-MPP8 antibody [EPR19856] (ab214812)](https://www.abcam.com/ps/products/214/ab214812/Images/ab214812-3-benefits-of-recombinant-antibodies.png)
Anti-MPP8 antibody [EPR19856] (ab214812)
