1,5-Anhydroglucitol Uptake Assay Kit (Cell-Based) (ab273330)
Key features and details
- Assay type: Cell-based (qualitative)
- Detection method: Fluorescent
- Platform: Flow cytometer, Fluorescence microscope
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
1,5-Anhydroglucitol Uptake Assay Kit (Cell-Based) -
Detection method
Fluorescent -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based (qualitative) -
Assay duration
Multiple steps standard assay -
Product overview
1,5-Anhydroglucitol Uptake Assay Kit (Cell-Based) (ab273330) utilizes a proprietary fluorescent 1,5-AG analog, which can be taken up by cells. However, this 1,5-AG analog cannot be fully utilized in glycolytic processes and thus accumulates inside the cells. Fluorescence generated by this analog is proportional to the cellular 1,5-AG uptake. The assay can be used to monitor 1,5-AG uptake by using fluorescent microscopy and flow cytometry. Phloretin, a compound that can inhibit glucose transporter and 1,5-AG cell uptake, is included in the kit as a control. This non-radioactive assay kit is easy-to-use and allows qualitative and quantitative measurements of 1,5-AG uptake in cultured cells
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Tested applications
Suitable for: Flow Cyt, FMmore details -
Platform
Flow cytometer, Fluorescence microscope
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 50 tests Analysis Buffer 1 x 1.8ml Phloretin (100X in DMSO) 1 x 75µl Reagent 1 x 200µl
Images
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Uptake of 1,5 AG in Jurkat cells
Flow Cytometry histograms comparing the inhibition of 1,5-AG uptake by Phloretin in Jurkat cells (Black: negative control cells; orange: Phloretin-treated cells; blue: untreated cells)
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1,5-AG uptake in Jurkat cellsFluorescence microscopy images of Jurkat cells. Top: Phloretin and 1,5-AG treated cells; Bottom: 1,5-AG treated cells.
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1,5-AG uptake in HeLa cells1,5-AG uptake in HeLa cells: HeLa cells showing the uptake of AGTracker Reagent in the cytoplasm. Cells were stained with Reagent for 30 min. and fixed. Image was taken using a fluorescent microscope with a 60X oil objective lens.