Hypochlorite Assay Kit (Colorimetric) (ab219929)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Lysate, Urine
- Sensitivity: 0.001 %
Overview
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Product name
Hypochlorite Assay Kit (Colorimetric)
See all Hypochlorite kits -
Detection method
Colorimetric -
Sample type
Urine, Serum, Plasma, Other biological fluids, Cell Lysate, Tissue Lysate -
Assay type
Quantitative -
Sensitivity
0.001 % -
Species reactivity
Reacts with: Other species -
Product overview
Hypochlorite Detection Kit (Colorimetric) (ab219929) provides a sensitive assay for measuring hypochlorite (HClO, hypochlorous acid) with high specificity in cell and tissue lysates and biological fluids. The hypochlorite sensor used in the assay selectively reacts with hypochlorite (hypochlorous) to generate a red color product which can be measured with an absorbance microplate reader at OD 555 nm.
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Notes
Hypochlorite anion (ClO-) and its protonated form, hypochlorous acid (HClO) are critical reactive oxygen species (ROS) in biological systems. Uncontrolled production of hypochlorite (hypochlorous acid) can lead to tissue damage and diseases including arthritis, renal failure and cancers. In addition, sodium hypochlorite (NaClO) has been widely used as a bleaching agent for surface cleaning, odor removal and water disinfection in our daily lives. Exposure to large amount of sodium hypochlorite can lead to poisoning with the symptoms of serious breathing problems, stomach irritation, redness and pain on skin and eye. Therefore, highly selective and sensitive detection of hypochlorite (hypochlorous acid) is of toxicological and environmental importance.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 200 tests Assay Buffer 1 x 20ml DMSO 1 x 600µl Hypochlorite Sensor 1 vial Hypochlorite Standard 1 x 300µl
Images
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Hypochlorite Detection Kit (Colorimetric) (ab219929)
Typical Hypochlorite standard calibration curve. Hypochlorite was measured with in a 96 well clear bottom plate. As low as 0.001% (10 ppm) sodium hypochlorite (NaClO) was detected with 3-5 minutes incubation.
