Hypochlorite Assay Kit (Fluorometric) (ab219930)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Lysate, Urine
- Sensitivity: 3 µM
Overview
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Product name
Hypochlorite Assay Kit (Fluorometric)
See all Hypochlorite kits -
Detection method
Fluorescent -
Sample type
Urine, Serum, Plasma, Other biological fluids, Cell Lysate, Tissue Lysate -
Assay type
Quantitative -
Sensitivity
3 µM -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Hypochlorite Detection Kit (Fluorimetric) (ab219930) provides a sensitive fluorescence-based assay for measuring hypochlorite (HClO, hypochlorous acid) with high specificity in cell and tissue lysates and biological fluids. Upon selective reaction with hypochlorite (hypochlorous), the weakly fluorescent Hypochlorite Sensor used in the assay selectively reacts with hypochlorite (hypochlorous) generates a strongly fluorescent product with more than 100-fold fluorescence enhancement. The fluorescence signal can be measured by a fluorescence microplate reader at Ex/Em = 540/590 nm. The assay can detect as low as 3 µM hypochlorite was detected in a 100 µL reaction volume.
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Notes
Hypochlorite anion (ClO-) and its protonated form, hypochlorous acid (HClO) are critical reactive oxygen species (ROS) in biological systems. Uncontrolled production of hypochlorite (hypochlorous acid) can lead to tissue damage and diseases including arthritis, renal failure and cancers. In addition, sodium hypochlorite (NaClO) has been widely used as a bleaching agent for surface cleaning, odor removal and water disinfection in our daily lives. Exposure to large amount of sodium hypochlorite can lead to poisoning with the symptoms of serious breathing problems, stomach irritation, redness and pain on skin and eye. Therefore, highly selective and sensitive detection of hypochlorite (hypochlorous acid) is of toxicological and environmental importance.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 200 tests Assay Buffer 1 x 20ml DMSO 1 x 100µl Hypochlorite Sensor 1 vial Hypochlorite Standard 1 x 300µl
Images
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Hypochlorite Detection Kit (Fluorimetric) (ab219930)
Typical Hypochlorite standard calibration curve. Hypochlorite was measured with in a 96 well solid black plate. As low as 0.003 mM (~3 µM) sodium hypochlorite (NaClO) was detected with 10-30 min incubation.
