Human WIF-1 ELISA Kit (ab267596)
Key features and details
- Sensitivity: 0.1 ng/ml
- Range: 0.102 ng/ml - 25 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human WIF-1 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.1 ng/ml -
Range
0.102 ng/ml - 25 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 86.25 72% - 107% Plasma 94.4 82% - 115% Cell culture media 133.9 108% - 143% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human WIF-1 ELISA Kit is designed for the quantitative determination of WIF-1 in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for human WIF-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and WIF-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human WIF-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of WIF-1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit shows no cross-reactivity with the following cytokines tested: Human 2B4, ADAM-9, APRIL, BMP-2, BMP-9, Cathepsin L, FABP2, FAP, FGF-19, Galectin-3, HGFR, IFN-alpha/beta R2, IGF-2R, IL-1R6, IL-24, IL-33, Kallikrein-14, Legumain, LOX-1, MBL, Neprilysin, Notch, NOV, Osteoactivin, PD-1, PGRPs, SERPINA4, sFRP-3, Thrombomodulin, TLR2, Transferrin.
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Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Anti-Human WIF-1 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Human WIF-1 Detection Antibody 2 vials Human WIF-1 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Binds to WNT proteins and inhibits their activities. May be involved in mesoderm segmentation. -
Sequence similarities
Contains 5 EGF-like domains.
Contains 1 WIF domain. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- WIF-1
- wif1
- WIF1_HUMAN
- Wnt inhibitory factor 1
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Database links
- Entrez Gene: 11197 Human
- Omim: 605186 Human
- SwissProt: Q9Y5W5 Human
- Unigene: 284122 Human
Images
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Example data
This standard curve is for demonstration only. A standard curve must be run with each assay.