Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cytokeratin 6 antibody [EPR1602Y] (ab93279)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1602Y] to Cytokeratin 6
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-Cytokeratin 6 antibody [EPR1602Y]
    See all Cytokeratin 6 primary antibodies

  • Description

    Rabbit monoclonal [EPR1602Y] to Cytokeratin 6

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Cytokeratin 6 aa 1-100 (N terminal). The exact sequence is proprietary. P48668, P04259 and P02538 100% homology.

  • Positive control

    • WB: HaCaT and A431 cell lysates, human skin and tonsil tissue lysates. IHC-P: Human tonsil and squamous cervical carcinoma tissues. ICC/IF: A431 cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Cytokeratin 6 antibody [EPR1602Y] (ab93279)
    Western blot - Anti-Cytokeratin 6 antibody [EPR1602Y] (ab93279)
    All lanes : Anti-Cytokeratin 6 antibody [EPR1602Y] (ab93279) at 1/2000 dilution (purified)

    Lane 1 : Human skin tissue lysate
    Lane 2 : Human tonsil tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 60 kDa
    Observed band size: 56 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 6 antibody [EPR1602Y] (ab93279)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 6 antibody [EPR1602Y] (ab93279)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling Cytokeratin 6 with purified ab93279 at 1/50. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500).

    Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 6 antibody [EPR1602Y] (ab93279)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 6 antibody [EPR1602Y] (ab93279)

    Immunocytochemistry/Immunofluorescence analysis of A431 (Human epidermoid carcinoma cell line) cells labelling Cytokeratin 6 with purified ab93279 at 1/50. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/500) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.

    Control 1: Primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 6 antibody [EPR1602Y] (ab93279)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 6 antibody [EPR1602Y] (ab93279)

    ICC/IF image of unpurified ab93279 stained A431 (Human epidermoid carcinoma cell line) cells.

    The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93279, 1/100 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabiit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Western blot - Anti-Cytokeratin 6 antibody [EPR1602Y] (ab93279)
    Western blot - Anti-Cytokeratin 6 antibody [EPR1602Y] (ab93279)
    Anti-Cytokeratin 6 antibody [EPR1602Y] (ab93279) at 1/10000 dilution (purified) + HaCaT (Human keratinocyte cell line) cell lysate at 20 µg

    Secondary
    Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 60 kDa
    Observed band size: 56 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Cytokeratin 6 antibody [EPR1602Y] (ab93279)
    Western blot - Anti-Cytokeratin 6 antibody [EPR1602Y] (ab93279)
    Anti-Cytokeratin 6 antibody [EPR1602Y] (ab93279) at 1/5000 dilution (unpurified) + A431 (Human epidermoid carcinoma cell line) cell lysate at 10 µg

    Predicted band size: 60 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 6 antibody [EPR1602Y] (ab93279)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 6 antibody [EPR1602Y] (ab93279)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human squamous cervical carcinoma tissue labelling Cytokeratin 6 with unpurified ab93279 at a dilution of 1/250.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-Cytokeratin 6 antibody [EPR1602Y] (ab93279)
    Anti-Cytokeratin 6 antibody [EPR1602Y] (ab93279)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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