Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Cytokeratin 6 antibody [EPR1603Y] (ab52620)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR1603Y] to Cytokeratin 6
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Human

Product name

Anti-Cytokeratin 6 antibody [EPR1603Y]
See all Cytokeratin 6 primary antibodies
Description

Rabbit monoclonal [EPR1603Y] to Cytokeratin 6
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide corresponding to Human Cytokeratin 6 aa 550-650 (C terminal). P48668, P02538 and P04259.
Database link: P48668
Positive control
- A431 cell lysate, human squamous cervical carcinoma, A431 cells.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR1603Y
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 6 antibody [EPR1603Y] (ab52620)

Ab52620 at 1/100-1/250 dilution staining human squamous cervical carcinoma; paraffin embedded.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 6 antibody [EPR1603Y] (ab52620)

Immunocytochemistry/Immunofluorescence analysis of HaCaT (Human keratinocyte cell line) labelling Cytokeratin 6 with purified ab52620 at 1/1000. Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.
Western blot - Anti-Cytokeratin 6 antibody [EPR1603Y] (ab52620)

Anti-Cytokeratin 6 antibody [EPR1603Y] (ab52620) at 1/100000 dilution + A431 cell lysate at 10 µg

Secondary
Goat anti-rabbit HRP antibody at 1/2000 dilution

Predicted band size: 60 kDa
Observed band size: 60 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 6 antibody [EPR1603Y] (ab52620)

Ab52620 at 1/100-1/250 dilution staining A431 cells.
Anti-Cytokeratin 6 antibody [EPR1603Y] (ab52620)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com