Human WISP-1 ELISA Kit (CCN4) (ab155445)
Key features and details
- Sensitivity: 50 pg/ml
- Range: 0.061 ng/ml - 15 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated anti-Human WISP-1 2 vials Recombinant Human WISP-1 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml WISP-1 Microplate (12 x 8 wells) 1 unit -
Research areas
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Relevance
WISP1 is a downstream regulator in the Wnt/Frizzled-signaling pathway and is associated with cell survival. WISP1 attentuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase and up-regulates the anti-apoptotic Bcl-X(L) protein. WISP1 is expressed in heart, kidney, lung, pancreas, placenta, ovary, small intestine and spleen. Overexpression is associated with several cancers including breast cancer and colon tumors. -
Cellular localization
Secreted -
Alternative names
- CCN 4
- CCN family member 4
- CCN4
see all -
Database links
- Entrez Gene: 8840 Human
- Omim: 603398 Human
- SwissProt: O95388 Human
- Unigene: 492974 Human
Images
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Typical Standard Curve
Representative standard curve using ab155445 - Assay Diluent B.
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Typical Standard CurveRepresentative standard curve using ab155445 - Assay Diluent C.
