Human GBA knockout HeLa cell pellet (ab278951)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit Human GBA knockout HeLa cell pellet 1 vial Human wild-type HeLa cell pellet 1 vial -
Research areas
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Cell type
epithelial -
Disease
Adenocarcinoma -
Gender
Female -
STR Analysis
Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8, 12 CSF1PO: 9, 10
Images
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Western blot - Human GBA knockout HeLa cell pellet (ab278951)Lane 1: Wild-type HeLa cell lysate (20µg)
Lane 2: GBA knockout HeLa cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab128879 observed at 60 kDa. Red - loading control ab8245 observed at 37 kDa.
ab128879 Anti-GBA antibody [EPR5143(3)] was shown to specifically react with GBA in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265038 (knockout cell lysate ab256929) was used. Wild-type and GBA knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab128879 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Western blot - Human GBA knockout HeLa cell pellet (ab278951)Lane 1: Wild-type HeLa cell lysate (20µg)
Lane 2: GBA knockout HeLa cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab125065 observed at 70 kDa. Red - loading control ab8245 observed at 37 kDa.
ab125065 Anti-GBA antibody [EPR5142] was shown to specifically react with GBA in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265038 (knockout cell lysate ab256929) was used. Wild-type and GBA knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab125065 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Sanger Sequencing - Human GBA knockout HeLa cell pellet (ab278951)Homozygous: 1 bp insertion in exon 3
