Mouse/Rat FGF1 ELISA Kit (ab223587)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 2 pg/ml
- Range: 16 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Serum, Tissue Lysate, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse, Rat
Overview
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Product name
Mouse/Rat FGF1 ELISA Kit
See all FGF1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Brain tissue 5 2.7% Inter-assay Sample n Mean SD CV% Brain tissue 3 3.7% -
Sample type
Cell culture supernatant, Urine, Serum, EDTA Plasma, Cit plasma, Tissue Lysate -
Assay type
Sandwich (quantitative) -
Sensitivity
2 pg/ml -
Range
16 pg/ml - 1000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine 111 95% - 135% Serum 116 111% - 119% Cell culture media 117 108% - 125% EDTA Plasma 105 102% - 109% Cit plasma 119 111% - 125% Tissue Lysate 118 105% - 126% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse, Rat -
Product overview
Mouse/Rat FGF1 ELISA Kit (ab223587) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FGF1 protein in cit plasma, edta plasma, serum, tissue lysate, urine, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse/Rat FGF1 with NULL NULL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
FGF1, also known as Fibroblast Growth Factor 1 or FGF acidic, is an important activator for several signaling cascades with roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. It functions as potent mitogen in vitro. In the presence of heparin, FGF1 binds in a 1:2 stoichiometry with FGF1 receptor protein, causing the autophosphorylation and activation of the receptor. This complex then binds to integrin and recruits PTPN11 to begin FGF1 signaling. Because of its possible role in organ development and in tumor growth, FGF1 is an important subject of study for the treatment of diabetes and the diagnosis and treatment of cancer. Mouse and rat FGF1 are identical on an amino acid level.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPR 1 x 6ml 10X Mouse/Rat FGF1 Capture Antibody 1 x 600µl 10X Mouse/Rat FGF1 Detector Antibody 1 x 600µl Mouse/Rat FGF1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 25BS 1 x 20ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
The heparin-binding fibroblast growth factors play important roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. They are potent mitogens in vitro. -
Sequence similarities
Belongs to the heparin-binding growth factors family. -
Cellular localization
Secreted. Cytoplasm. Cytoplasm > cell cortex. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1. - Information by UniProt
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Alternative names
- Acidic fibroblast growth factor
- aFGF
- Beta endothelial cell growth factor
see all -
Database links
- Entrez Gene: 14164 Mouse
- Entrez Gene: 25317 Rat
- SwissProt: P61148 Mouse
- SwissProt: P61149 Rat
- Unigene: 241282 Mouse
- Unigene: 88013 Rat
Images
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Other - Mouse/Rat FGF1 ELISA Kit (ab223587)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse/rat FGF1 standard curve in 1X Cell Extraction Buffer PTR.Background-subtracted data values (mean +/- SD) are graphed.
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Example of mouse/rat FGF1 standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Example of mouse/rat FGF1 standard curve in Sample Diluent 25BS.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of spiked mouse/rat FGF1 in mouse serum, plasma and cell culture supernatant samples.The concentrations of FGF1 were measured in duplicates, interpolated from the FGF1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 25% and plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Interpolated concentrations of spiked mouse/rat FGF1 in rat serum and plasma samples.The concentrations of FGF1 were measured in duplicates, interpolated from the FGF1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, plasma (EDTA) 50%, and RAW 264.7 supernatant 95%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Interpolated concentrations of native mouse/rat FGF1 in mouse and rat brain tissue extract.Interpolated concentrations of native mouse/rat FGF1 in mouse and rat brain tissue extract based on a 31 or 63 μg/mL extract load, respectively. The concentrations of FGF1 were measured in duplicate and interpolated from the FGF standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FGF1 concentration was determined to be 697 pg/mL in mouse brain tissue extract and 796 pg/mL in rat brain tissue extract.
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Interpolated concentrations of spiked FGF1 in mouse and rat urine.The concentrations of FGF1 were measured in duplicate and interpolated from the FGF1 standard curve and corrected for sample dilution. Undiluted samples are as follows: mouse urine 25%, rat urine 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Human FGF1 active protein (hFGF1) was added at the indicated concentrations to 500 pg/ml mouse FGF1 (mFGF1) to test for Interference.Only 14% interference was observed with the highest dose of hFGF1.
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Sandwich ELISA - Mouse/Rat FGF1 ELISA Kit (ab223587)To learn more about the advantages of recombinant antibodies see here.
