Anti-P Glycoprotein antibody [JSB-1] (ab3366)
![Anti-P Glycoprotein antibody [JSB-1] (ab3366)](https://www.abcam.com/ps/products/3/ab3366/Images/ab3366-7505-anti-p-glycoprotein-antibody-jsb-1-immunohistochemistry.jpg "Anti-P Glycoprotein antibody [JSB-1] (ab3366)")
Key features and details
- Mouse monoclonal [JSB-1] to P Glycoprotein
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-P Glycoprotein antibody [JSB-1]
See all P Glycoprotein primary antibodies -
Description
Mouse monoclonal [JSB-1] to P Glycoprotein -
Host species
Mouse -
Specificity
This antibody is specific for the MDR1 isoform of P Glycoprotein which is only found in the plasma membrane. It does not cross-react with MDR3. It has been shown to cross-react with Pyruvate Carboxylase, an abundant mitochondrial enzyme (130 kDa), on both immunoblots and immunohistochemical tissue sections. Weak homogeneous, cytoplasmic, or granular patterns of reactivity may represent staining of the PC cross-reactive epitope rather than positive staining for P glycoprotein. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Multidrug-resistant Chinese hamster ovary cell line (CHrC5).
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Epitope
Cytoplasmic domain present on the MDR1 isoform of P Glycoprotein. This epitope is strongly conserved in mammals. -
Positive control
- Drug-sensitive parental cell lines and their multidrug-resistant derivatives. Human liver (positive staining detected along luminal surfaces of bilecanaliculi) or human colon (positive staining localized to luminal surface of secretaoryepithelium).
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General notes
This antibody is reported to work in WB according to the literature(PubMed 22389470 and 17961285). Due to customer feedback of difficulty in WB, we are no longer able to guarantee ab3366 in WB.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
JSB-1 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P Glycoprotein antibody [JSB-1] (ab3366)ab3366 (4µg/ml) staining p Glycoprotein in human liver, using an automated system (DAKO Autostainer Plus). Using this protocol there is a cell membrane staining of a subpopulation of hepatocytes.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
