Anti-Flotillin 2/ESA antibody [EPR14128(B)] - BSA and Azide free (ab250555)
![Anti-Flotillin 2/ESA antibody [EPR14128(B)] - BSA and Azide free (ab250555)](https://www.abcam.com/ps/products/250/ab250555/Images/ab250555-395496-anti-flotillin-2esa-antibody-epr14128-western-blot-human-knockout-lysate.jpg "Anti-Flotillin 2/ESA antibody [EPR14128(B)] - BSA and Azide free (ab250555)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14128(B)] to Flotillin 2/ESA - BSA and Azide free
- Suitable for: IP, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Flotillin 2/ESA antibody [EPR14128(B)] - BSA and Azide free
See all Flotillin 2/ESA primary antibodies -
Description
Rabbit monoclonal [EPR14128(B)] to Flotillin 2/ESA - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK293T, HeLa and A549 cell lysates.
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General notes
Ab250555 is the carrier-free version of ab181988. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250555 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14128(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Flotillin 2/ESA antibody [EPR14128(B)] - BSA and Azide free (ab250555)All lanes : Anti-Flotillin 2/ESA antibody [EPR14128(B)] (ab181988) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : FLOT2 knockout HEK293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab181988).
Lanes 1-4: Merged signal (red and green). Green - ab181988 observed at 47 kDa. Red - loading control ab8245 observed at 36 kDa.
ab181988 Anti-Flotillin 2/ESA antibody [EPR14128(B)] was shown to specifically react with Flotillin 2/ESA in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266847 (knockout cell lysate ab257442) was used. Wild-type and Flotillin 2/ESA knockout samples were subjected to SDS-PAGE. ab181988 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Anti-Flotillin 2/ESA antibody [EPR14128(B)] - BSA and Azide free (ab250555)](https://www.abcam.com/ps/products/250/ab250555/Images/ab250555-2-benefits-of-recombinant-antibodies.png)
Anti-Flotillin 2/ESA antibody [EPR14128(B)] - BSA and Azide free (ab250555)
