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Human TLR4 ELISA Kit (ab277392)

Key features and details

  • Sensitivity: 0.4 ng/well
  • Range: 0.41 ng/ml - 100 ng/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human TLR4 ELISA Kit

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall
    Inter-assay
    Sample n Mean SD CV%
    Overall

  • Sample type

    Cell culture supernatant, Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    0.4 ng/well

  • Range

    0.41 ng/ml - 100 ng/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 103.8 87% - 121%
    Plasma 91.18 68% - 109%
    Cell culture media 113.1 107% - 122%

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human TLR4 ELISA Kit (ab277392) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TLR4 in serum (human TLR4 concentration is low in normal serum and plasma samples, and may not be detectable in this assay), plasma and cell culture supernatants.


    This assay employs an antibody specific for Human TLR4 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human TLR4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human TLR4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human TLR4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    300X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent 1 x 15ml
    Biotinylated Anti-Human TLR4 Antibody 2 vials
    Anti-Human TLR4 coated Microplate 1 unit
    Human TLR4 standard protein (Lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml

  • Research areas

  • Function

    Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS-independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific.

  • Tissue specificity

    Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells.

  • Involvement in disease

    Genetic variation in TLR4 is associated with age-related macular degeneration type 10 (ARMD10) [MIM:611488]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.

  • Sequence similarities

    Belongs to the Toll-like receptor family.
    Contains 18 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 TIR domain.

  • Domain

    The TIR domain mediates interaction with NOX4.

  • Post-translational
    modifications

    N-glycosylated. Glycosylation of Asn-526 and Asn-575 seems to be necessary for the expression of TLR4 on the cell surface and the LPS-response. Likewise, mutants lacking two or more of the other N-glycosylation sites were deficient in interaction with LPS.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Alternative names

    • ARMD10
    • CD284
    • CD284 antigen
    • Homolog of Drosophila toll
    • hToll
    • TLR 4
    • TLR4
    • TLR4_HUMAN
    • TOLL
    • Toll like receptor 4
    • Toll-like receptor 4
    see all

  • Database links

    Images

    • Example data
      Example data

      These standard curves are for demonstration only. A standard curve must be run with each assay.

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