Antibodies, Proteins, Kits and Reagents for Life Science

324 988 ₸ Product size

100 µl 324 988 ₸ 10 µl 130 665 ₸

Order now and get it on Tuesday March 09, 2021

Anti-pro Caspase-7 antibody [Y33] (ab32067)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [Y33] to pro Caspase-7
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Human

Product name

Anti-pro Caspase-7 antibody [Y33]
See all pro Caspase-7 primary antibodies
Description

Rabbit monoclonal [Y33] to pro Caspase-7
Host species

Rabbit
Specificity

The antibody only recognizes the pro-form of Caspase-7. It does not react with the cleaved forms (active enzyme) of Caspase-7.
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Jurkat cell lysate and human lung squamous carcinoma.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Clonality

Monoclonal
Clone number

Y33
Isotype

IgG
Research areas

Western blot - Anti-pro Caspase-7 antibody [Y33] (ab32067)

Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: Staurosporin treated HAP1 whole cell lysate (20 µg)
Lane 3: CASP7 knockout HAP1 whole cell lysate (20 µg)
Lane 4: Staurosporin treated CASP7 knockout HAP1 whole cell lysate (20 µg)
Lane 5: HeLa whole cell lysate (20 µg)
Lane 6: Staurosporin treated HeLa whole cell lysate (20 µg)

Lanes 1 - 6: Merged signal (red and green). Green - ab32067 observed at 38 kDa. Red - loading control, ab18058, observed at 130 kDa.

ab32067 was shown to recognize pro Caspase 7 when HAP1 + pro Caspase 7 knockout samples were used, along with additional cross-reactive bands. Wild-type and pro Caspase 7 knockout samples were subjected to SDS-PAGE. Ab32067 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pro Caspase-7 antibody [Y33] (ab32067)

Immunohistochemical analysis of paraffin-embedded human lung squamous carcinoma using ab32067 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-pro Caspase-7 antibody [Y33] (ab32067)

Anti-pro Caspase-7 antibody [Y33] (ab32067) at 1/1000 dilution + Jurkat cell lysate

Predicted band size: 34 kDa
Observed band size: 34 kDa
Anti-pro Caspase-7 antibody [Y33] (ab32067)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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