Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-pro Caspase-7 antibody [Y33] (ab32067)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y33] to pro Caspase-7
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-pro Caspase-7 antibody [Y33]
    See all pro Caspase-7 primary antibodies

  • Description

    Rabbit monoclonal [Y33] to pro Caspase-7

  • Host species

    Rabbit

  • Specificity

    The antibody only recognizes the pro-form of Caspase-7. It does not react with the cleaved forms (active enzyme) of Caspase-7.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Jurkat cell lysate and human lung squamous carcinoma.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-pro Caspase-7 antibody [Y33] (ab32067)
    Western blot - Anti-pro Caspase-7 antibody [Y33] (ab32067)

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: Staurosporin treated HAP1 whole cell lysate (20 µg)
    Lane 3: CASP7 knockout HAP1 whole cell lysate (20 µg)
    Lane 4: Staurosporin treated CASP7 knockout HAP1 whole cell lysate (20 µg)
    Lane 5: HeLa whole cell lysate (20 µg)
    Lane 6: Staurosporin treated HeLa whole cell lysate (20 µg)

    Lanes 1 - 6: Merged signal (red and green). Green - ab32067 observed at 38 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab32067 was shown to recognize pro Caspase 7 when HAP1 + pro Caspase 7 knockout samples were used, along with additional cross-reactive bands. Wild-type and pro Caspase 7 knockout samples were subjected to SDS-PAGE. Ab32067 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pro Caspase-7 antibody [Y33] (ab32067)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pro Caspase-7 antibody [Y33] (ab32067)

    Immunohistochemical analysis of paraffin-embedded human lung squamous carcinoma using ab32067 at 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-pro Caspase-7 antibody [Y33] (ab32067)
    Western blot - Anti-pro Caspase-7 antibody [Y33] (ab32067)
    Anti-pro Caspase-7 antibody [Y33] (ab32067) at 1/1000 dilution + Jurkat cell lysate

    Predicted band size: 34 kDa
    Observed band size: 34 kDa

  • Anti-pro Caspase-7 antibody [Y33] (ab32067)
    Anti-pro Caspase-7 antibody [Y33] (ab32067)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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