Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Tuesday March 09, 2021

Anti-INSL3 antibody [EPR20739] (ab227974)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20739] to INSL3
Suitable for: WB, IHC-P, IP
Reacts with: Human

Product name

Anti-INSL3 antibody [EPR20739]
See all INSL3 primary antibodies
Description

Rabbit monoclonal [EPR20739] to INSL3
Host species

Rabbit
Specificity

This product recognises a different epitope compared to ab224643.

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human testis lysate. IHC-P: Human testis, ovary and Leydig tumor tissues. IP: Human testis lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20739
Isotype

IgG
Research areas

Western blot - Anti-INSL3 antibody [EPR20739] (ab227974)

Anti-INSL3 antibody [EPR20739] (ab227974) at 1/10000 dilution + Human testis lysate at 10 µg

Secondary
VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

Developed using the ECL technique.

Predicted band size: 15 kDa
Observed band size: 15 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INSL3 antibody [EPR20739] (ab227974)

Immunohistochemical analysis of paraffin-embedded human testis tissue labeling INSL3 with ab227974 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in Leydig cells of human testis is observed (PMID: 19329805). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INSL3 antibody [EPR20739] (ab227974)

Immunohistochemical analysis of paraffin-embedded human Leydig tumor tissue labeling INSL3 with ab227974 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in human Leydig tumor cells is observed (PMID: 25609776). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-INSL3 antibody [EPR20739] (ab227974)

All lanes : Anti-INSL3 antibody [EPR20739] (ab227974) at 1/1000 dilution

Lane 1 : Untreated human testis lysate
Lane 2 : Human testis lysate treated with double DTT (100 mmol/L)

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Developed using the ECL technique.

Predicted band size: 15 kDa
Observed band size: 15,30 kDa
why is the actual band size different from the predicted?

Exposure time: 1 second

Bocking/Dilution buffer: 5% NFDM/TBST.

The 30kDa band is a dimer.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INSL3 antibody [EPR20739] (ab227974)

Immunohistochemical analysis of paraffin-embedded human ovary tissue labeling INSL3 with ab227974 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in thecal cells of human ovary is observed (PMID: 21138583). Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-INSL3 antibody [EPR20739] (ab227974)

INSL3 was immunoprecipitated from 0.35 mg of human testis lysate with ab227974 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab227974 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: Human testis lysate 10 µg (Input).

Lane 2: ab227974 IP in human testis lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab227974 in human testis lysate.

Exposure time: 10 seconds.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Anti-INSL3 antibody [EPR20739] (ab227974)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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