Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-INSL3 antibody [EPR20739] (ab227974)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20739] to INSL3
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-INSL3 antibody [EPR20739]
    See all INSL3 primary antibodies

  • Description

    Rabbit monoclonal [EPR20739] to INSL3

  • Host species

    Rabbit

  • Specificity

    This product recognises a different epitope compared to ab224643.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human testis lysate. IHC-P: Human testis, ovary and Leydig tumor tissues. IP: Human testis lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR20739

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-INSL3 antibody [EPR20739] (ab227974)
    Western blot - Anti-INSL3 antibody [EPR20739] (ab227974)
    Anti-INSL3 antibody [EPR20739] (ab227974) at 1/10000 dilution + Human testis lysate at 10 µg

    Secondary
    VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

    Developed using the ECL technique.

    Predicted band size: 15 kDa
    Observed band size: 15 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INSL3 antibody [EPR20739] (ab227974)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INSL3 antibody [EPR20739] (ab227974)

    Immunohistochemical analysis of paraffin-embedded human testis tissue labeling INSL3 with ab227974 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in Leydig cells of human testis is observed (PMID: 19329805). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INSL3 antibody [EPR20739] (ab227974)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INSL3 antibody [EPR20739] (ab227974)

    Immunohistochemical analysis of paraffin-embedded human Leydig tumor tissue labeling INSL3 with ab227974 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in human Leydig tumor cells is observed (PMID: 25609776). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-INSL3 antibody [EPR20739] (ab227974)
    Western blot - Anti-INSL3 antibody [EPR20739] (ab227974)
    All lanes : Anti-INSL3 antibody [EPR20739] (ab227974) at 1/1000 dilution

    Lane 1 : Untreated human testis lysate
    Lane 2 : Human testis lysate treated with double DTT (100 mmol/L)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 15 kDa
    Observed band size: 15,30 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 second


    Bocking/Dilution buffer: 5% NFDM/TBST.

    The 30kDa band is a dimer.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INSL3 antibody [EPR20739] (ab227974)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-INSL3 antibody [EPR20739] (ab227974)

    Immunohistochemical analysis of paraffin-embedded human ovary tissue labeling INSL3 with ab227974 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in thecal cells of human ovary is observed (PMID: 21138583). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-INSL3 antibody [EPR20739] (ab227974)
    Immunoprecipitation - Anti-INSL3 antibody [EPR20739] (ab227974)

    INSL3 was immunoprecipitated from 0.35 mg of human testis lysate with ab227974 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab227974 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Human testis lysate 10 µg (Input). 

    Lane 2: ab227974 IP in human testis lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab227974 in human testis lysate.

     

    Exposure time: 10 seconds.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  • Anti-INSL3 antibody [EPR20739] (ab227974)
    Anti-INSL3 antibody [EPR20739] (ab227974)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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