Anti-INSL3 antibody [EPR14834(2)] (ab199536)
![Anti-INSL3 antibody [EPR14834(2)] (ab199536)](https://www.abcam.com/ps/products/199/ab199536/Images/ab199536-242166-anti-insl3-antibody-epr148342-western-blot.jpg "Anti-INSL3 antibody [EPR14834(2)] (ab199536)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14834(2)] to INSL3
- Suitable for: IP, WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-INSL3 antibody [EPR14834(2)]
See all INSL3 primary antibodies -
Description
Rabbit monoclonal [EPR14834(2)] to INSL3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human and Mouse testis lysates. IP: Human testis whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14834(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-INSL3 antibody [EPR14834(2)] (ab199536)All lanes : Anti-INSL3 antibody [EPR14834(2)] (ab199536) at 1/5000 dilution
Lane 1 : Mouse testis lysate
Lane 2 : Human testis lysate
Lane 3 : Human fetal heart lysate
Lane 4 : Human fetal kidney lysate
Lane 5 : Human fetal spleen lysate
Lane 6 : NCCIT (Human pluripotent embryonal carcinoma) whole cell lysate
Lane 7 : A431 (Human epidermoid carcinoma) whole cell lysate
Lane 8 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 15 kDa
Observed band size: 15 kDa
Exposure time: 3 minutesINSL3 is expressed in prenatal and postnatal Leydig cells. Human fetal heart, kidney, spleen, NCCIT, A431 & Jurkat cells represent negative controls in this experiment.
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-INSL3 antibody [EPR14834(2)] (ab199536)](https://www.abcam.com/ps/products/199/ab199536/Images/ab199536-242165-anti-insl3-antibody-epr148342-immunoprecipitation.jpg)
Immunoprecipitation - Anti-INSL3 antibody [EPR14834(2)] (ab199536)INSL3 was immunoprecipitated from 1mg of Human testis whole cell lysate with ab199536 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab199536 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: Human testis whole cell lysate 10 µg (Input). Lane 2: ab199536 IP in Human testis whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199536 in Human testis whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
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![Anti-INSL3 antibody [EPR14834(2)] (ab199536)](https://www.abcam.com/ps/products/199/ab199536/Images/ab199536-4-benefits-of-recombinant-antibodies.png)
Anti-INSL3 antibody [EPR14834(2)] (ab199536)
