Canine IGFBP2 ELISA Kit (ab273184)
Key features and details
- Sensitivity: 0.055 ng/ml
- Range: 0.051 ng/ml - 12.5 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Dog
Overview
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Product name
Canine IGFBP2 ELISA Kit
See all IGFBP2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.055 ng/ml -
Range
0.051 ng/ml - 12.5 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 105.3 97% - 112% Plasma 98.77 91% - 104% Cell culture media 87.73 74% - 104% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Dog -
Product overview
Canine IGFBP2 ((Insulin-like Growth Factor Binding Protein 2) ELISA Kit (ab273184) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Canine IGFBP2 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Canine IGFBP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and Canine IGFBP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Canine IGFBP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Canine IGFBP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 550X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Anti-Canine IGFBP2 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Canine IGFBP2 Antibody 2 vials Canine IGFBP2 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Inhibits IGF-mediated growth and developmental rates. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. -
Sequence similarities
Contains 1 IGFBP N-terminal domain.
Contains 1 thyroglobulin type-1 domain. -
Domain
The C-terminus is required for IGF-binding and growth inhibition. -
Post-translational
modificationsO-glycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- BP 2
- BP2
- IBP 2
see all
Images
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Canine IGFBP2 ELISA Kit standard curve
This standard curve is for demonstration only. A standard curve must be run with each assay.