Anti-HURP antibody (ab70744)
Key features and details
- Rabbit polyclonal to HURP
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HURP antibody
See all HURP primary antibodies -
Description
Rabbit polyclonal to HURP -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to a region between residue 800 and the C-terminus (residue 846) of Human HURP (NP_055565.2).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-HURP antibody (ab70744)Detection of HURP by Western Blot of Immunprecipitate. ab70744 at 1µg/ml staining HURP in HeLa whole cell lysate immunoprecipitated using ab70744 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Chemiluminescence with exposure time of 10 seconds. -
Western blot - Anti-HURP antibody (ab70744)All lanes : Anti-HURP antibody (ab70744) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 94 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
