Human SPARCL1 ELISA Kit (SPARC-like protein 1) (ab272478)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 7.89 pg/ml
- Range: 39.06 pg/ml - 2500 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human SPARCL1 ELISA Kit (SPARC-like protein 1)
See all SPARCL1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 3.3% Inter-assay Sample n Mean SD CV% Serum 3 3.27% -
Sample type
Serum, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
7.89 pg/ml -
Range
39.06 pg/ml - 2500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 92 89% - 95% Tissue Extracts 105 104% - 106% Hep Plasma 99 93% - 108% EDTA Plasma 99 96% - 104% Cit plasma 102 92% - 116% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human SPARCL1 ELISA kit (ab272478) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human SPARCL1 protein in serum, plasma and cell and tissue culture extract. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human SPARCL1 with 7.89 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
SPARCL1 (SPARC-Like Protein 1), also known as HEVIN, is a 75 kD protein that is encoded by the SPARCL1 gene. SPARCL1 is highly expressed in high endothelial venules (HEVs) and is thought to contribute to the induction or maintenance of features of the HEV endothelium that facilitate lymphocyte migration. SPARCL1 is also highly expressed in heart, lung, brain, skeletal muscle, ovary, colon, and small intestine tissues. SPARCL1 may have implications in the regulation of cell adhesion, migration and proliferation. The antibody pair was designed against the mature full-length extracellular fragment of SPARCL1. SPARCL1 shares 64%, 64%, 72%, and 93% sequence homology in mouse, rat, cow, and monkey, respectively.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human SPARCL1 (SPARC-like protein 1) Capture Antibody 1 x 600µl 10X Human SPARCL1 (SPARC-like protein 1) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human SPARCL1 (SPARC-like protein 1) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Tissue specificity
Highly expressed in lymph node, brain, heart, lung, skeletal muscle, ovary, small intestine, and colon, with lower levels in placenta, pancreas, testis, spleen, and thymus, and no expression in kidney, liver, and peripheral blood leukocytes. -
Sequence similarities
Belongs to the SPARC family.
Contains 1 EF-hand domain.
Contains 1 follistatin-like domain.
Contains 1 Kazal-like domain. -
Post-translational
modificationsN- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. Thr-398 is the preferred site to Thr-399. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Alternative names
- Ecm2
- Extracellular matrix protein 2
- Hevin
see all -
Database links
- Entrez Gene: 8404 Human
- Omim: 606041 Human
- SwissProt: Q14515 Human
- Unigene: 62886 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human SPARCL1 standard curve in Sample Diluent NS. -
Interpolated concentrations of native SPARCL1 in human serum and plasma samples.The concentrations of SPARCL1 were measured in duplicates, interpolated from the SPARCL1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:100, plasma (citrate) 1:200, plasma (EDTA) 1:100, and plasma (heparin) 1:200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SPARCL1 concentration was determined to be 215.28 ng/mL in serum, 468.22 ng/mL in plasma (citrate), 231.62 ng/mL in plasma (EDTA), and 428.58 ng/mL in plasma (heparin).
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Interpolated concentrations of native SPARCL1 in human brain tissuebased on a 25 μg/mL extract load and human lung based on a 250 μg/mL extract load. The concentrations of SPARCL1 were measured in duplicate and interpolated from the SPARCL1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SPARCL1 concentration was determined to be 519.79 pg/mL in brain extract and 108.09 pg/mL in lung extract.
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Sandwich ELISA - Human SPARCL1 ELISA Kit (SPARC-like protein 1) (ab272478)To learn more about the advantages of recombinant antibodies see here.
