Human SFRP1 ELISA Kit (ab277082)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 18.5 pg/ml
- Range: 54.69 pg/ml - 3500 pg/ml
- Sample type: Cell culture media, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human SFRP1 ELISA Kit
See all SFRP1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 4.1% Inter-assay Sample n Mean SD CV% Serum 3 4% -
Sample type
Serum, Cell culture media, Hep Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
18.5 pg/ml -
Range
54.69 pg/ml - 3500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 87 80% - 98% Cell culture media 96 92% - 102% Hep Plasma 86 85% - 87% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human SFRP1 ELISA kit (ab277082) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human SFRP1 protein in human serum and plasma - heparin. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human SFRP1 with 18.5 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Soluble frizzled-related protein 1 (SFRP1) is part of a family of SFRPs that function as modulators of Wnt signaling through direct interaction with Wnts. SFRPs have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels, has antiproliferative effects on vascular cells and can induce in vivo, an angiogenic response. In vascular cell cycle, SFRP1 delays the G1 phase and entry into the S phase. In kidney development, it inhibits tubule formation and bud growth in metanephroi. Additionally, it Inhibits WNT1/WNT4-mediated TCF-dependent transcription. SFRP1 shares 96.8%, 95.8%, 97.8%, and 98.2% sequence homology with mouse, rat, dog, and bovine, respectively.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Human SFRP1 Capture Antibody 1 x 600µl 10X Human Human SFRP1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human Human SFRP1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels (By similarity). Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase (By similarity). In kidney development, inhibits tubule formation and bud growth in metanephroi (By similarity). Inhibits WNT1/WNT4-mediated TCF-dependent transcription. -
Tissue specificity
Widely expressed. Absent from lung, liver and peripheral blood leukocytes. Highest levels in heart and fetal kidney. Also expressed in testis, ovary, fetal brain and lung, leiomyomal cells, myometrial cells and vascular smooth muscle cells. Expressed in foreskin fibroblasts and in keratinocytes. -
Sequence similarities
Belongs to the secreted frizzled-related protein (sFRP) family.
Contains 1 FZ (frizzled) domain.
Contains 1 NTR domain. -
Domain
The FZ domain is involved in binding with Wnt ligands. -
Cellular localization
Secreted. Cell membrane or extracellular matrix-associated. Released by heparin-binding. - Information by UniProt
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Alternative names
- Frizzled related protein 1
- FRP
- FRP 1
see all -
Database links
- Entrez Gene: 6422 Human
- Omim: 604156 Human
- SwissProt: Q8N474 Human
- Unigene: 213424 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human SFRP1 standard curve in Sample Diluent NS.The SFRP1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native SFRP1 in human serum and plasma samples.The concentrations of SFRP1 were measured in duplicates, interpolated from the SFRP1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SFRP1 concentration was determined to be 287 pg/mL in serum and 487 pg/mL in plasma (heparin).
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Sandwich ELISA - Human SFRP1 ELISA KitTo learn more about the advantages of recombinant antibodies see here.
