Human PDGF-CC Antibody Pair - BSA and Azide free (ab244114)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Human
Overview
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Product name
Human PDGF-CC Antibody Pair - BSA and Azide free
See all PDGF CC kits -
Assay type
ELISA set -
Range
28.125 pg/ml - 1800 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
The Antibody Pair can be used to quantify Human PDGF-CC. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab234568), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Human PDGF-CC Capture Antibody (unconjugated) 1 x 100µg Human PDGF-CC Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Relevance
The platelet-derived growth factor (PDGF) family of heparin-binding growth factors consists of five known members, denoted PDGF-AA, PDGF-BB, PDGF-AB, PDGF-CC and PDGF-DD. The mature and active form of these proteins, an anti-parallel disulfide-linked dimer of two 12-14 kDa polypeptide chains, is obtained through proteolytic processing of biologically inactive precursor proteins, which contain an N-terminal CUB domain and a PDGF/VEGF homologous domain. The PDGFs interact with two related protein tyrosine kinase receptors, PDGFR-alpha and PDGFR-beta, and are potent mitogens for a variety of cell types, including smooth muscle cells, connective tissue cells, bone and cartilage cells, and certain tumor cells. They play an important role in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubules epithelial cell development. Mature PDGFs are stored in platelet alpha-granules and are released upon platelet activation. PDGF-AA, -AB, -BB and –CC signal primarily through the PDGF R alpha receptor, whereas PDGF-DD interacts almost exclusively with the PDGF R beta receptor. -
Cellular localization
Cytoplasmic, Nuclear, Secreted and Cytoplasmic granule -
Alternative names
- FALLOTEIN
- PDGF C
- PDGFC
see all -
Database links
- Entrez Gene: 56034 Human
- Omim: 608452 Human
- SwissProt: B9EGR8 Human
- SwissProt: Q9NRA1 Human
Images
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Sandwich ELISA - Human PDGF-CC Antibody Pair - BSA and Azide free (ab244114)To learn more about the advantages of recombinant antibodies see here.
