Human Obestatin ELISA Kit (ab264628)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 8 pg/ml
- Range: 23.44 pg/ml - 1500 pg/ml
- Sample type: EDTA Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Obestatin ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Plasma 8 3% -
Sample type
EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
8 pg/ml -
Range
23.44 pg/ml - 1500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range EDTA Plasma 96 % - % -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Obestatin ELISA Kit (ab264628) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Obestatin protein in edta plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Obestatin with 8 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Obestatin, is generated from the proteolytic cleavage of preproghrelin; its sequence corresponds to amino acids 76-98 of Appetite-regulating hormone [Precursor](Q9QYH7). This peptide has contrasting actions to Ghrelin (a different product of proteolytic cleavage of preproghrelin) in that it inhibits food intake and gastrointestinal motility but does not modify in vitro GH release from pituitary cells.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Obestatin Capture Antibody 1 x 600µl 10X Human Obestatin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human Obestatin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
Obestatin, is generated from the proteolytic cleavage of preproghrelin; its sequence corresponds to aamino acids 76-98 of Appetite-regulating hormone [Precursor](Q9QYH7). This peptide has contrasting actions to Ghrelin (a different product of proteolytic cleavage of preproghrelin) in that it inhibits food intake and gastrointestinal motility but does not modify in vitro GH release from pituitary cells. -
Cellular localization
Secreted -
Alternative names
- Appetite-regulating hormone
- Growth hormone secretagogue
- Growth hormone-releasing peptide
see all -
Database links
- Entrez Gene: 51738 Human
- Omim: 605353 Human
- SwissProt: Q9UBU3 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Obestatin standard curve in Sample Diluent NS.The Obestatin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Obestatin in human plasma (EDTA) samples.The concentrations of Obestatin were measured in duplicates, interpolated from the Obestatin standard curves and corrected for sample dilution. Undiluted samples are as follows: plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Obestatin concentration was determined to be 289 pg/mL in plasma (EDTA).
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Sandwich ELISA - Human Obestatin ELISA Kit (ab264628)To learn more about the advantages of recombinant antibodies see here.
