Human Obestatin Antibody Pair - BSA and Azide free (ab267733)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
Overview
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Product name
Human Obestatin Antibody Pair - BSA and Azide free -
Assay type
ELISA set -
Range
23.438 ng/ml - 1500 ng/ml -
Product overview
The Antibody Pair can be used to quantify Human Obestatin. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab264628), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Human Obestatin Capture Antibody (unconjugated) 1 x 100µg Human Obestatin Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Relevance
Obestatin is a peptide hormone. This relatively small protein (~3 kDa) is encoded by the same gene that also encodes ghrelin, also a peptide hormone. The protein produced by that gene breaks into two smaller peptides, ghrelin and obestatin by enzyme cleavage. Obestatin has been found to suppress appetite whereas ghrelin increases it. Obestatin is produced in the cells that line the stomach and small intestines. Obestatin results in decreased food intake and reduces the gastric emptying activities. It is mainly expressed in the GI tract with higher expressions in the stomach. Obestatin is an endogenous ligand for the orphan G protein-coupled receptor GPR39. -
Cellular localization
Secreted -
Alternative names
- Appetite regulating hormone
- ghrelin/obestatin preprohormone
- GHRL
see all
Images
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Sandwich ELISA - Human Obestatin Antibody Pair - BSA and Azide free (ab267733)To learn more about the advantages of recombinant antibodies see here.
