Human Myostatin ELISA kit (ab267656)
Key features and details
- Sensitivity: 0.83 ng/ml
- Range: 0.819 ng/ml - 200 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Myostatin ELISA kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.83 ng/ml -
Range
0.819 ng/ml - 200 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 101.9 86% - 123% Plasma 89.4 67% - 122% Cell culture media 102 99% - 107% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human Myostatin ELISA Kit is designed for the quantitative determination of Myostatin in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for human Myostatin coated on a 96-well plate. Standards and samples are pipetted into the wells and Myostatin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Myostatin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Myostatin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
This ELISA kit shows no cross-reactivity with the following cytokines tested: Human ACE, Activin RIB (ALK-4), ADAM23, Artemin, Cardiotrophin-1 (CT-1), Cathepsin V, FABP1 (LFABP), FGF-20, HAI-1, IL-27 R alpha (WSX-1), Insulin R (CD220), Kallikrein 7, LIF R alpha, Lipocalin-1, LTbR (Lymphotoxin beta R), Mesothelin, MFRP, Neuropilin-2, Neurturin, Nidogen-2, Olfactomedin-2 (Noelin-2), p53, PDECGF (Thymidine Phosphorylase), PDGF-CC, Progranulin, Ret, ROBO4, Semaphorin 6B, Serpin F1 (PEDF), SREC-I (SCARF1), SREC-II (SCARF2), TLR1, TLR3, TPP1 (Tripeptidyl-peptidase I), TREM-2, TrkC, TROY (TNFRSF19), Uromodulin, XIAP.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Anti-Human Myostatin coated Microplate (12 x 8 wells) 1 unit Assay Diluent A 1 x 30ml Biotinylated Anti-Human Myostatin Detection Antibody 2 vials Human Myostatin Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Acts specifically as a negative regulator of skeletal muscle growth. -
Sequence similarities
Belongs to the TGF-beta family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- GDF 8
- GDF-8
- GDF8
see all -
Database links
- Entrez Gene: 2660 Human
- Omim: 601788 Human
- SwissProt: O14793 Human
- Unigene: 41565 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay