Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant human GDF8 / Myostatin protein (ab50097)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Endotoxin level:
  • Active: Yes
  • Suitable for: Inhibition Assay, SDS-PAGE, Functional Studies

Description

  • Product name

    Recombinant human GDF8 / Myostatin protein
    See all GDF8 / Myostatin proteins and peptides

  • Biological activity

    Determined by its ability to inhibit the proliferation of MPC-11 cells. The expected ED50 for this effect is 17.0-25.0 ng/ml.

  • Purity

    > 95 % SDS-PAGE.
    Greater than 98% by SDS-PAGE and HPLC analyses.

  • Endotoxin level

  • Expression system

    Escherichia coli

  • Accession

    O14793

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      DFGLDCDEHS TESRCCRYPL TVDFEAFGWD WIIAPKRYKA NYCSGECEFV FLQKYPHTHL VHQANPRGSA GPCCTPTKMS PINMLYFNGK EQIIYGKIPA MVVDRCGCS

    • Predicted molecular weight

      25 kDa

    • Amino acids

      267 to 375

    • Associated products

      Specifications

      Our Abpromise guarantee covers the use of ab50097 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      • Applications

        Inhibition Assay

        SDS-PAGE

        Functional Studies

      • Form

        Lyophilized
      • Concentration information loading...

      Preparation and Storage

      • Stability and Storage

        Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

        This product is an active protein and may elicit a biological response in vivo, handle with caution.

      • Reconstitution
        Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4oC for 1 week or -20oC for future use.

      General Info

      • Alternative names

        • GDF 8
        • GDF-8
        • GDF8
        • GDF8_HUMAN
        • Growth differentiation factor 8
        • Growth/differentiation factor 8
        • MSLHP
        • MSTN
        • Myostatin
        • OTTHUMP00000163498
        see all

      • Function

        Acts specifically as a negative regulator of skeletal muscle growth.

      • Sequence similarities

        Belongs to the TGF-beta family.

      • Cellular localization

        Secreted.
      • Information by UniProt

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab50097? Please let us know so that we can cite the reference in this datasheet.

    ab50097 has not yet been referenced specifically in any publications.

    Preparation and Storage

    • Alternative names

      • GDF 8
      • GDF-8
      • GDF8
      • GDF8_HUMAN
      • Growth differentiation factor 8
      • Growth/differentiation factor 8
      • MSLHP
      • MSTN
      • Myostatin
      • OTTHUMP00000163498
      see all

    • Function

      Acts specifically as a negative regulator of skeletal muscle growth.

    • Sequence similarities

      Belongs to the TGF-beta family.

    • Cellular localization

      Secreted.
    • Information by UniProt

    Images

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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