11-dehydro-TXB2 ELISA Kit (ab133054)
Key features and details
- Sensitivity: 4.31 pg/ml
- Range: 9.8 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Urine
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
Overview
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Product name
11-dehydro-TXB2 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Low conc 16 67pg/ml 13.2% Medium conc 16 165pg/ml 15.3% High conc 16 318pg/ml 8.1% Inter-assay Sample n Mean SD CV% Low conc 8 72pg/ml 17.5% Medium conc 8 216pg/ml 12.6% High conc 8 1250pg/ml 16.3% -
Sample type
Cell culture supernatant, Urine -
Assay type
Competitive -
Sensitivity
4.31 pg/ml -
Range
9.8 pg/ml - 10000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Urine 112.2 % - % Tissue Culture Media 104.8 % - % -
Assay time
3h 0m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human
Predicted to work with: a wide range of other species
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Product overview
Abcam’s 11-dehydro-TXB2in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 11-dehydro-TXB2 in tissue culture media and urine.
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-11-dehydro-TXB2 antigen and a polyclonal rabbit antibody specific to 11-dehydro-TXB2. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of 11-dehydro-TXB2 captured in the plate.
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Notes
11-dehydro-Thromboxane B2 (11-dehydro-TXB2) is a major metabolite of Thromboxane B2 (TXB2) found in urine and plasma. It is produced by the dehydrogenation of the alcohol group on the C11 position of TXB2 by the enzyme 11-OH-dehydrogenase. The detection of 11-dehydro-TXB2 is often used to measure thromboxane production in vivo. Monitoring levels of this metabolite is a helpful tool in the study of several diseases, such as liver cirrhosis, cystic fibrosis, mastocytosis, systemic lupus erythematosus, thrombosis diseases, and other diseases involving platelet activation.11-dehydro-TXB2 has also been used in diabetes and asthma studies.
Cross reactivities:
Compound Cross Reactivity 11-dehydro-TXB2 100% PGE2 1.85% TXB2 0.4% PGF2α 0.2% 6-keto-PGF1α 0.17% PGD2 0.1% 2,3-dinor TXB2 0.1% 8-iso-PGF2α 0.07% 11-b-PGF2α 0.05% -
Platform
Microplate
Properties
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Storage instructions
Please refer to protocols. -
Components 1 x 96 tests 11-dehydro-Thromboxane B2 Standards 1 x 500µl 11-dehydro-TXB2 Alkaline Phosphatase Conjugate 1 x 6ml 11-dehydro-TXB2 Antibody 1 x 6ml 20X Wash Buffer Concentrate 1 x 30ml Assay Buffer 1 x 30ml Goat anti-rabbit IgG Microplate (12 x 8 wells) 1 unit Plate Sealer 1 unit pNpp Substrate 1 x 20ml Stop Solution 1 x 5ml -
Research areas
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Alternative names
- 11-dehydro-Thromboxane B2
Images
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Typcial Standard Curve
Representative Standard Curve using ab133054.
