Thromboxane B2 ELISA Kit (ab133022)
Key features and details
- Sensitivity: 10.54 pg/ml
- Range: 13.7 pg/ml - 10000 pg/ml
- Sample type: Hep Plasma, Saliva, Serum, Tissue Culture Media, Urine
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
Overview
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Product name
Thromboxane B2 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Low 24 759pg/ml = 3.6% Medium 24 1283pg/ml = 4% High 24 2605pg/ml = 1.6% Inter-assay Sample n Mean SD CV% Low 8 44pg/ml = 7.6% Medium 8 371pg/ml = 3.6% High 8 3053pg/ml = 6.2% -
Sample type
Saliva, Urine, Serum, Tissue Culture Media, Hep Plasma -
Assay type
Competitive -
Sensitivity
= 10.54 pg/ml -
Range
13.7 pg/ml - 10000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Saliva = 98.3 % - % Urine = 102.2 % - % Serum = 113 % - % Tissue Culture Media = 104.7 % - % Hep Plasma = 111 % - % -
Assay time
3h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human
Predicted to work with: a wide range of other species
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Product overview
Abcam’s Thromboxane B2 (TXB2) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Thromboxane B2 in biological fluids.
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-TXB2 antigen and a rabbit polyclonal antibody specific to TXB2. After incubation the excess reagents are washed away and pNpp substrate is added. After a short incubation the enzyme reaction is stopped and a yellow color is generated. The intensity of the yellow coloration is inversely proportional to the amount of TXB2 captured in the plate.
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Notes
Thromboxane A2 (TXA2) is involved in platelet aggregation, vasoconstriction and reproductive functions, but has a half life of only 37 seconds under physiological conditions. Thromboxane B2 (TXB2) is the stable product of the non-enzymatic hydration of TXA2, thus the production of TXA2 in vivo is typically monitored by measurement of TXB2 and its metabolite 2,3-dinor TXB2. 11-dehydro-TXB2 is a major metabolite of Thromboxane B2 (TXB2) found in urine and plasma produced by its dehydrogenation by the enzyme 11-OH-dehydrogenase.
Cross Reactivity
Compound Cross Reactivity TXB2 100% 2,3-dinorTXB2 7.1% 11-dehydro TXB2 0.4% 6-keto-PGF1α 0.23% PGD2 PGE2 PGF1α PGF2α -
Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 27ml Assay Buffer 1 x 27ml Goat anti-rabbit IgG Microplate (12 x 8 wells) 1 unit Plate Sealer 1 unit pNpp Substrate 1 x 20ml Rabbit Polyclonal Thromboxane B2 Antibody 1 x 5ml Stop Solution 1 x 5ml Thromboxane B2 Alkaline Phosphatase Conjugate 1 x 5ml Thromboxane B2 Standard 1 x 500µl -
Research areas
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Alternative names
- T box 2
- T box transcription factor TBX2
- T-box protein 2
see all
Images
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Typical Standard Curve
Representative Standard Curve using ab133022.