Anti-CD147 antibody (ab232967)
Key features and details
- Rabbit polyclonal to CD147
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CD147 antibody
See all CD147 primary antibodies -
Description
Rabbit polyclonal to CD147 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human CD147 aa 50-300. Two N-terminal tags, His-tag and GST-tag. Expressed in E.coli.
Database link: P35613-1 -
Positive control
- IHC-P: Human prostate cancer, kidney, glioma, stomach, stomach cancer, colorectal cancer tissue. ICC/IF: HeLa cells. WB: Recombinant human CD147 protein; HeLa cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-CD147 antibody (ab232967)Anti-CD147 antibody (ab232967) at 2 µg/ml + Recombinant human CD147 protein
Secondary
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD147 antibody (ab232967)Formalin-fixed, paraffin-embedded human colorectal cancer tissue stained for CD147 using ab232967 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Immunocytochemistry/ Immunofluorescence - Anti-CD147 antibody (ab232967)HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling CD147 using ab232967 at 20 µg/ml in ICC/IF. FITC staining.
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Western blot - Anti-CD147 antibody (ab232967)Anti-CD147 antibody (ab232967) at 2 µg/ml + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Secondary
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD147 antibody (ab232967)Formalin-fixed, paraffin-embedded human stomach cancer tissue stained for CD147 using ab232967 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD147 antibody (ab232967)Formalin-fixed, paraffin-embedded human stomach tissue stained for CD147 using ab232967 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD147 antibody (ab232967)Formalin-fixed, paraffin-embedded human glioma tissue stained for CD147 using ab232967 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD147 antibody (ab232967)Formalin-fixed, paraffin-embedded human kidney tissue stained for CD147 using ab232967 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD147 antibody (ab232967)Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for CD147 using ab232967 at 10 µg/ml in immunohistochemical analysis.
Secondary used: HRP-Linked Goat anti-Rabbit IgG polyclonal at 2 µg/ml. DAB staining.
