Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

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Anti-BMP2 antibody [EPR20807] (ab214821)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20807] to BMP2
Suitable for: WB, ICC/IF, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-BMP2 antibody [EPR20807]
See all BMP2 primary antibodies
Description

Rabbit monoclonal [EPR20807] to BMP2
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Mouse
ICC/IF	Mouse Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Caco-2, Saos-2, C2C12, RAW 264.7, C6 and LNCaP whole cell lysates; Mouse cartilage tissue lysate; Rat spleen tissue lysate; Human lung cancer and fetal kidney tissue lysates. ICC/IF: LNCaP and RAW 264.7 cells. Flow Cyt: RAW 264.7 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20807
Isotype

IgG
Research areas

Western blot - Anti-BMP2 antibody [EPR20807] (ab214821)

All lanes : Anti-BMP2 antibody [EPR20807] (ab214821) at 1/1000 dilution

Lane 1 : Caco-2 (human colorectal adenocarcinoma cell line) whole cell lysate
Lane 2 : Saos-2 (human osteosarcoma cell line) whole cell lysate
Lane 3 : C2C12 (mouse myoblast cell line) whole cell lysate
Lane 4 : RAW264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG H&L (HRP))

Developed using the ECL technique.

Predicted band size: 44 kDa
Observed band size: 14,18,30 kDa
why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure times: Lane 1: 3 minutes; Lanes 2-4: 15 seconds.

The molecular weight observed is consistent with literature. Bands observed at 14KD are monomers (PMID: 12819188, PMID: 3200834). Bands observed at 18KD are glycosylated monomers (PMID: 12819188, PMID: 8117284, PMID: 16261447, PMID: 3200834). Bands observed at ~30KD are dimers or glycosylated dimers) (PMID: 22508088, PMID: 16261447, PMID: 3200834).
Immunocytochemistry/ Immunofluorescence - Anti-BMP2 antibody [EPR20807] (ab214821)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized LNCaP (human prostate cancer cell line) cells labeling BMP2 with ab214821 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on LNCaP cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Flow Cytometry - Anti-BMP2 antibody [EPR20807] (ab214821)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell line labeling BMP2 with ab214821 at 1/700 dilution (red) compared with an Isotype control Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.
Western blot - Anti-BMP2 antibody [EPR20807] (ab214821)

All lanes : Anti-BMP2 antibody [EPR20807] (ab214821) at 1/1000 dilution

Lane 1 : His-tagged human BMP2 (aa283-395) recombinant protein
Lane 2 : His-tagged human BMP4 (aa293-408) recombinant protein

Lysates/proteins at 0.02 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG H&L (HRP))

Developed using the ECL technique.

Predicted band size: 44 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?

Exposure time: 1 second

Blocking and dilution buffer: 5% NFDM/TBST
Immunocytochemistry/ Immunofluorescence - Anti-BMP2 antibody [EPR20807] (ab214821)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labeling BMP2 with ab214821 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on RAW 264.7 cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Western blot - Anti-BMP2 antibody [EPR20807] (ab214821)

All lanes : Anti-BMP2 antibody [EPR20807] (ab214821) at 1/1000 dilution

Lane 1 : LNCaP (human prostate cancer cell line) whole cell lysate at 20 µg
Lane 2 : Mouse cartilage tissue lysate at 20 µg
Lane 3 : Human lung cancer tissue lysate at 20 µg
Lane 4 : Human fetal kidney tissue lysate at 20 µg
Lane 5 : Rat spleen tissue lysate at 10 µg
Lane 6 : C6 (rat glial tumor cell line) whole cell lysate at 10 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG H&L (HRP))

Developed using the ECL technique.

Predicted band size: 44 kDa
Observed band size: 14,18,30 kDa why is the actual band size different from the predicted?

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure times: Lanes 1-4: 3 minutes; Lane 5: 30 seconds; Lane 6: 5 seconds.

The molecular weight observed is consistent with literature. Bands observed at 14KD are monomers (PMID: 12819188, PMID: 3200834). Bands observed at 18KD are glycosylated monomers (PMID: 12819188, PMID: 8117284, PMID: 16261447, PMID: 3200834). Bands observed at ~30KD are dimers or glycosylated dimers) (PMID: 22508088, PMID: 16261447, PMID: 3200834).
Anti-BMP2 antibody [EPR20807] (ab214821)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BMP2 antibody [EPR20807] (ab214821)

Key features and details

Overview

Product name

Description

Host species

Tested Applications & Species

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Images

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Recombinant human BMP2 protein (Animal Free) (ab255993)