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Human LRIG3 ELISA Kit (ab267624)

Key features and details

  • Sensitivity: 2.4 ng/ml
  • Range: 2.4 ng/ml - 800 ng/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human LRIG3 ELISA Kit

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    overall
    Inter-assay
    Sample n Mean SD CV%
    overall

  • Sample type

    Cell culture supernatant, Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    2.4 ng/ml

  • Range

    2.4 ng/ml - 800 ng/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 106.5 75% - 132%
    Plasma 83.8 72% - 89%
    Cell culture media 142.7 136% - 146%

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human LRIG3 ELISA Kit is designed for the quantitative determination of LRIG3 in cell culture supernatants, plasma and serum samples.


    This assay employs an antibody specific for human LRIG3 coated on a 96-well plate. Standards and samples are pipetted into the wells and LRIG3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human LRIG3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of LRIG3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    This ELISA antibody pair detects Human LRIG3. Other species not determined.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    900X HRP-Streptavidin Concentrate 1 x 200µl
    Anti-Human LRIG3 coated Microplate (12 x 8 wells) 1 unit
    Assay Diluent C 1 x 30ml
    Biotinylated Anti-Human LRIG3 Detection Antibody 2 vials
    Human LRIG3 Standard (Lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Solution 1 x 12ml

  • Research areas

  • Function

    May play a role in craniofacial and inner ear morphogenesis during embryonic development. May act within the otic vesicle epithelium to control formation of the lateral semicircular canal in the inner ear, possibly by restricting the expression of NTN1.

  • Tissue specificity

    Widely expressed.

  • Sequence similarities

    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 15 LRR (leucine-rich) repeats.
    Contains 1 LRRCT domain.
    Contains 1 LRRNT domain.

  • Cellular localization

    Cell membrane. Cytoplasmic vesicle membrane. Detected in cytoplasmic vesicles when coexpressed with ERBB4.
  • Information by UniProt

  • Alternative names

    • Leucine-rich repeats and immunoglobulin-like domains protein 3
    • Leucine-rich repeats- and immunoglobulin-like domains- containing protein 3
    • LIG-3
    • LIG3
    • LRIG1-3
    • Lrig3
    • LRIG3_HUMAN
    see all

  • Database links

    Images

    • Human LRIG3 ELISA kit standard curve
      Human LRIG3 ELISA kit standard curve

      This standard curve is for demonstration only. A standard curve must be run with each assay.

       

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