Trypsin Activity Assay Kit (Colorimetric) (ab102531)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 2 hr
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
Overview
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Product name
Trypsin Activity Assay Kit (Colorimetric)
See all PRSS1 kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
Assay type
Enzyme activity -
Range
10 mU/well - 100 mU/well -
Assay time
2h 00m -
Species reactivity
Reacts with: Mammals -
Product overview
Trypsin Activity Assay Kit (Colorimetric) ab102531 is simple and fast assay to quantify trypsin activity.
In the trypsin activity assay protocol, trypsin cleaves a substrate to generate p-nitroaniline (p-NA) which is detected at OD = 405 nm. Since the color intensity is proportional to p-NA content, trypsin activity can be accurately measured.
The trypsin activity assay kit can detect 10-100 mU (p-NA unit) trypsin.
Trypsin activity assay protocol summary:
- add samples, positive control and standards to wells
- add chymotrypsin inhibitor to sample and positive control wells and incubate for 10 min at room temp
- add reaction mix
- analyze every 2-3 min for 1-2 hr with microplate reader in kinetic mode at 25ºC -
Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Chymotrypsin Inhibitor (TPCK,10 mM) White 1 x 100 units p-NA Standard (2 mM) Yellow 1 x 400µl Positive Control ( lyophilized) Blue 1 vial Trypsin Assay Buffer WM 1 x 25ml Trypsin Inhibitor (TLCK, 20 mM) Purple 1 x 100µl Trypsin Substrate (in DMSO) Red 1 x 200µl -
Research areas
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Function
Has activity against the synthetic substrates Boc-Phe-Ser-Arg-Mec, Boc-Leu-Thr-Arg-Mec, Boc-Gln-Ala-Arg-Mec and Boc-Val-Pro-Arg-Mec. The single-chain form is more active than the two-chain form against all of these substrates. -
Involvement in disease
Defects in PRSS1 are a cause of hereditary pancreatitis (HPC) [MIM:167800]; also known as chronic pancreatitis (CP). HPC is an autosomal dominant disease characterized by the presence of calculi in pancreatic ducts. It causes severe abdominal pain attacks. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsOccurs in a single-chain form and a two-chain form, produced by proteolytic cleavage after Arg-122. -
Cellular localization
Secreted > extracellular space. - Information by UniProt
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Alternative names
- Alpha-trypsin chain 2
- Beta-trypsin
- Cationic trypsinogen
see all
Images
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Functional Studies - Trypsin Activity Detection Kit (ab102531)
p-NA standard curve measured in duplicates ((+/- SD), background subtracted) after 60 minutes incubation.
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Functional Studies - Trypsin Activity Detection Kit (ab102531)Example of standard curve obtained using ab102531, following assay protocol. The curve should be used as guideline only.
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Functional Studies - Trypsin Activity Detection Kit (ab102531)Trypsin levels were kinetically measured in commercially purchased trypsin (1x); background signal subtracted (duplicates +/- SD).
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Functional Studies - Trypsin Activity Colorimetric Assay Kit (ab102531)Example of Trypsin sample timeline obtained using ab102531