Human IL-12 p70 ELISA Kit (ab223592)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 13.6 pg/ml
- Range: 31.3 pg/ml - 2000 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IL-12 p70 ELISA Kit
See all IL-12 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 5 7% Inter-assay Sample n Mean SD CV% Serum 3 5.2% -
Sample type
Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
13.6 pg/ml -
Range
31.3 pg/ml - 2000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 96 93% - 101% Hep Plasma 85 80% - 89% EDTA Plasma 99 97% - 101% Cit plasma 93 92% - 94% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human IL-12 p70 ELISA Kit (ab223592) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-12 p70 protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-12 p70 with 13.6 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
IL-12 p70 is a 70kDa cytokine heterodimer made of 2 subunits IL-12A (p35) and IL-12B (p40). This kit recognizes specifically the IL-12 total heterodimer protein. IL-12 p70 is produced by active antigen-presenting cells and promotes the development of Th1 and induces IFN?.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml 10X Human IL-12 p70 Capture Antibody 1 x 600µl 10X Human IL-12 p70 Detector Antibody 1 x 600µl Human IL-12 p70 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC. -
Sequence similarities
Belongs to the IL-6 superfamily. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- CLMF
- CLMF p35
- CLMF p40
see all -
Database links
- Entrez Gene: 3592 Human
- Entrez Gene: 3593 Human
- Omim: 161561 Human
- Omim: 161560 Human
- SwissProt: P29459 Human
- SwissProt: P29460 Human
- Unigene: 673 Human
Images
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Other - Human IL-12 p70 ELISA Kit (ab223592)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of Human IL-12 standard curveRaw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of spike IL-12 in human serum, plasma and cell culture supernatant samples.The concentrations of IL-12 p70 were measured in duplicates, interpolated from the IL-12 p70 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (EDTA) 50%, plasma (heparin) 2.5%, plasma (citrate) 50% and cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Serum from ten individual healthy human female donors was measured in duplicate. No detectable levels of IL-12 p70 were measured.
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Sandwich ELISA - Human IL-12 p70 ELISA Kit (ab223592)To learn more about the advantages of recombinant antibodies see here.
