Human IL-13R alpha 2 ELISA Kit (ab274400)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 96.3 pg/ml
- Range: 281.3 pg/ml - 18000 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cell Lysate, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IL-13R alpha 2 ELISA Kit
See all IL-13 receptor alpha 2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Supernatant 8 3.1% Inter-assay Sample n Mean SD CV% Supernatant 3 5.41% -
Sample type
Cell culture supernatant, Serum, Cell culture extracts, Cell Lysate, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
96.3 pg/ml -
Range
281.3 pg/ml - 18000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 104 103% - 105% Serum 86 83% - 92% Cell culture extracts 107 104% - 110% Hep Plasma 88 86% - 93% EDTA Plasma 91 90% - 94% Cit plasma 80 78% - 82% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human IL-13R alpha 2 ELISA kit (ab274400) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human IL-13R alpha 2 protein in serum, plasma cell culture supernatant, and cell and tissue extract. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-13R alpha 2 with 96.3 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Interleukin-13 receptor alpha 2 is one of the receptors for IL-13, which is a T helper 2 molecule involved in immune response, inflammation, and wound healing. Studies have shown overexpression of IL-13R alpha 2 can confer invasive and metastatic ability in variety of cancer cells such as melanoma, ovarian, pancreatic, colorectal, and gliomas. Therefore, making IL-13R alpha 2 a viable candidate as a prognostic biomarker. This kit is developed to recognize and target only IL-13R alpha 2.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human IL-13R alpha 2 Capture Antibody 1 x 600µl 10X Human IL-13R alpha 2 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human IL-13R alpha 2 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Binds as a monomer with high affinity to interleukin-13 (IL13), but not to interleukin-4 (IL4). -
Sequence similarities
Belongs to the type I cytokine receptor family. Type 5 subfamily.
Contains 1 fibronectin type-III domain. -
Domain
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- Cancer/testis antigen 19
- CD213a2
- CT19
see all -
Database links
- Entrez Gene: 3598 Human
- Omim: 300130 Human
- SwissProt: Q14627 Human
- Unigene: 336046 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human IL-13R alpha 2 standard curve in Sample Diluent NS.The IL-13R alpha 2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native IL-13R alpha 2 in human cell culture supernatant sample.The concentrations of IL-13R alpha 2 were measured in duplicates, interpolated from the IL-13R alpha 2 standard curves and corrected for sample dilution. Undiluted samples are as follows: A375 supernatant neat. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-13R alpha 2 concentration was determined to be 3,741 pg/mL in A375 supernatant.
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Interpolated concentrations of native IL-13R alpha 2 in human U-87MG and A375 samples based on a 400 µg/mL extract load.The concentrations of IL-13R alpha 2 were measured in duplicate and interpolated from the IL-13R alpha 2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-13R alpha 2 concentration was determined to be 8,147 pg/mL in U-87MG cell extract and 10,842 pg/mL in A375 cell extract.
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Sandwich ELISA - Human IL-13R alpha 2 ELISA Kit (ab274400)To learn more about the advantages of recombinant antibodies see here.
