Human HSPG ELISA Kit (Perlecan) (ab274393)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 47.215 pg/ml
- Range: 109.38 pg/ml - 7000 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human HSPG ELISA Kit (Perlecan)
See all Heparan Sulfate Proteoglycan 2/Perlecan kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 6.5% Inter-assay Sample n Mean SD CV% Serum 3 9.4% -
Sample type
Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
47.215 pg/ml -
Range
109.38 pg/ml - 7000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 89 83% - 95% Hep Plasma 107 103% - 113% EDTA Plasma 93 85% - 98% Cit plasma 102 97% - 107% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human HSPG ELISA kit (ab274393) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human HSPG protein in human serum and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human HSPG with 47.215 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
HSPG2 functions as an integral component of basement membranes. In the glomerular basement membrane, it provides a barrier that is both size- and charge-selective. HSPG2 serves as an attachment substrate for cells and plays essential roles in vascularization. It is critical for normal heart development and for regulating the vascular response to injury. HSPG2 in an anti-angiogenic and anti-tumor peptide that inhibits blood vessel growth in the chorioallantoic membrane. It blocks endothelial cell adhesion to fibronectin and type I collagen. Interaction with its ligand, integrin alpha2/beta1, is required for the anti-angiogenic properties.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human HSPG (Perlecan) Capture Antibody 1 x 600µl 10X Human HSPG (Perlecan) Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human HSPG (Perlecan) Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Integral component of basement membranes. Component of the glomerular basement membrane (GBM), responsible for the fixed negative electrostatic membrane charge, and which provides a barrier which is both size- and charge-selective. It serves as an attachment substrate for cells. Plays essential roles in vascularization. Critical for normal heart development and for regulating the vascular response to injury. Also required for avascular cartilage development.
Endorepellin in an anti-angiogenic and anti-tumor peptide that inhibits endothelial cell migration, collagen-induced endothelial tube morphogenesis and blood vessel growth in the chorioallantoic membrane. Blocks endothelial cell adhesion to fibronectin and type I collagen. Anti-tumor agent in neovascularization. Interaction with its ligand, integrin alpha2/beta1, is required for the anti-angiogenic properties. Evokes a reduction in phosphorylation of receptor tyrosine kinases via alpha2/beta1 integrin-mediated activation of the tyrosine phosphatase, PTPN6.
The LG3 peptide has anti-angiogenic properties that require binding of calcium ions for full activity. -
Tissue specificity
Found in the basement membranes. -
Involvement in disease
Defects in HSPG2 are the cause of Schwartz-Jampel syndrome (SJS1) [MIM:255800]; a rare autosomal recessive disorder characterized by permanent myotonia (prolonged failure of muscle relaxation) and skeletal dysplasia, resulting in reduced stature, kyphoscoliosis, bowing of the diaphyses and irregular epiphyses.
Defects in HSPG2 are the cause of dyssegmental dysplasia Silverman-Handmaker type (DDSH) [MIM:224410]. The dyssegmental dysplasias are rare, autosomal recessive skeletal dysplasias with anisospondyly and micromelia. There are two recognized types: the severe, lethal DDSH and the milder Rolland-Desbuquois form. Individuals with DDSH also have a flat face, micrognathia, cleft palate and reduced joint mobility, and frequently have an encephalocoele. The endochondral growth plate is short, the calcospherites (which are spherical calcium-phosphorus crystals produced by hypertrophic chondrocytes) are unfused, and there is mucoid degeneration of the resting cartilage. -
Sequence similarities
Contains 4 EGF-like domains.
Contains 22 Ig-like C2-type (immunoglobulin-like) domains.
Contains 11 laminin EGF-like domains.
Contains 3 laminin G-like domains.
Contains 3 laminin IV type A domains.
Contains 4 LDL-receptor class A domains.
Contains 1 SEA domain. -
Post-translational
modificationsProteolytic processing produces the C-terminal angiogenic peptide, endorepellin. This peptide can be further processed to produce the LG3 peptide.
N- and O-glycosylated; contains three heparan sulfate chains. The LG3 peptide contains at least three and up to five potential O-glycosylation sites but no N-glycosylation. -
Cellular localization
Secreted > extracellular space > extracellular matrix > basement membrane. - Information by UniProt
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Alternative names
- Basement membrane specific heparan sulfate proteoglycan core protein
- Endorepellin (domain V region)
- Heparan sulfate proteoglycan of basement membrane
see all -
Database links
- Entrez Gene: 3339 Human
- Omim: 142461 Human
- SwissProt: P98160 Human
- Unigene: 562227 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human HSPG2 standard curve in Sample Diluent NS.The HSPG2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native HSPG2 in human serum, plasma samplesThe concentrations of HSPG2 were measured in duplicates, interpolated from the HSPG2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 15%, plasma (citrate) 15%, plasma (EDTA) % and plasma (Heparin) 15%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean HSPG2 concentration was determined to be 39.48 ng/mL in serum, 27.14 ng/mL in plasma (citrate) and 76.72 ng/mL in plasma (EDTA) and 14.99 ng/ml in plasma (heparin)
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Sandwich ELISA - Human HSPG ELISA Kit (Perlecan) (ab274393)To learn more about the advantages of recombinant antibodies see here.
