Human MDC ELISA Kit (ab223866)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.3 pg/ml
- Range: 3.1 pg/ml - 200 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human, Rhesus monkey
Overview
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Product name
Human MDC ELISA Kit
See all MDC kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 5 2.8% Inter-assay Sample n Mean SD CV% Serum 3 2.1% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.3 pg/ml -
Range
3.1 pg/ml - 200 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 111 102% - 121% Serum 108 103% - 115% Cell culture media 100 98% - 102% Hep Plasma 78 60% - 88% EDTA Plasma 89 79% - 106% Cit plasma 118 111% - 131% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human, Rhesus monkey
Does not react with: Cow -
Product overview
Human MDC ELISA Kit (ab223866) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MDC protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MDC with 1.3 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Macrophage-derived chemokine (MDC), also known as C-C motif chemokine 22 (CCL22), is a secreted cytokine and member of the intercrine beta (chemokine CC) family. It is highly expressed in macrophage and in monocyte-derived dendritic cells. MDC may play a role in the trafficking of activated/effector T-lymphocytes to inflammatory sites and other aspects of activated T-lymphocyte physiology. MDC is a chemotactic for monocytes, dendritic cells and natural killer cells and potent chemoattractant for chronically activated T-lymphocytes.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human MDC Capture Antibody 1 x 600µl 10X Human MDC Detector Antibody 1 x 600µl Human MDC Lyophilized Recombinant Protein 2 vials Antibody Diluent 4BI 1 x 6ml 10X Wash Buffer PT (ab206977) 1 x 20ml TMB Development Solution 1 x 12ml Stop Solution 1 x 12ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Plate Seals 1 unit -
Research areas
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Function
May play a role in the trafficking of activated/effector T-lymphocytes to inflammatory sites and other aspects of activated T-lymphocyte physiology. Chemotactic for monocytes, dendritic cells and natural killer cells. Mild chemoattractant for primary activated T-lymphocytes and a potent chemoattractant for chronically activated T-lymphocytes but has no chemoattractant activity for neutrophils, eosinophils, and resting T-lymphocytes. Binds to CCR4. Processed forms MDC(3-69), MDC(5-69) and MDC(7-69) seem not be active. -
Tissue specificity
Highly expressed in macrophage and in monocyte-derived dendritic cells, and thymus. Also found in lymph node, appendix, activated monocytes, resting and activated macrophages. Lower expression in lung and spleen. Very weak expression in small intestine. In lymph node expressed in a mature subset of Langerhans' cells (CD1a+ and CD83+). Expressed in Langerhans' cell histiocytosis but not in dermatopathic lymphadenopathy. Expressed in atopic dermatitis, allergic contact dermatitis skin, and psoriasis, in both the epidermis and dermis. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Post-translational
modificationsThe N-terminal processed forms MDC(3-69), MDC(5-69) and MDC(7-69) are produced by proteolytic cleavage after secretion from monocyte derived dendrocytes. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- A 152E5.1
- ABCD 1
- ABCD1
see all -
Database links
- Entrez Gene: 6367 Human
- Omim: 602957 Human
- SwissProt: O00626 Human
- Unigene: 534347 Human
Images
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Other - Human MDC ELISA Kit (ab223866)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human MDC standard curve comparison dataStandard curve comparison between human MDC SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of human MDC standard curve in Sample Diluent NS.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native MDC in human serum, plasma and cell culture supernatant samples.The concentrations of MDC were measured in duplicates, interpolated from the MDC standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, plasma (heparin) 45%, plasma (EDTA) 25%, and 2 days 1.5% PHA-treated PBMC supernatant 4%. The interpolated, dilution factor-corrected values are plotted (mean +/- SD, n=2). The mean MDC concentration in neat sample was determined to be 678 pg/mL in serum, 844 pg/mL in plasma (citrate), 435 pg/mL in plasma (heparin), 781 pg/ml in plasma (EDTA), and 4352 pg/ml in PHA-treated PBMC supernatant.
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Serum from ten individual healthy human male donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MDC concentration was determined to be 698 pg/mL with a range of 376 – 967 pg/mL.
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Comparison of MDC in unstimulated and PHA stimulated PBMC cell supernatants.PBMC cells were cultured for 46 hours in the presence or absence of 1.5% PHA. The concentrations of MDC were measured in three dilutions of supernatant samples in duplicates and interpolated from the MDC standard curve. The interpolated values are plotted (mean +/- SD, n=3). The mean MDC concentration was determined to be 4519 pg/mL in neat PHA stimulated PBMC cell culture supernatant and 27 pg/mL in neat unstimulated PBMC cell culture supernatant.
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Serial dilutions of recombinant human 67 amino acid long MDCSerial dilutions of recombinant human 67 amino acid long MDC (aa27-93) were prepared and assayed in parallel with 69 amino acid long MDC (aa25-93), the standard of this kit.
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Sandwich ELISA - Human MDC ELISA Kit (ab223866)To learn more about the advantages of recombinant antibodies see here.
