Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Laminin beta 1 antibody [EPR3189(2)] - BSA and Azide free (ab247686)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3189(2)] to Laminin beta 1 - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Laminin beta 1 antibody [EPR3189(2)] - BSA and Azide free
    See all Laminin beta 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR3189(2)] to Laminin beta 1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa, JAR, A431, SW480, 293T and C6 cell lysates.

  • General notes

    Ab247686 is the carrier-free version of ab108536. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247686 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Laminin beta 1 antibody [EPR3189(2)] - BSA and Azide free (ab247686)
    Western blot - Anti-Laminin beta 1 antibody [EPR3189(2)] - BSA and Azide free (ab247686)
    All lanes : Anti-Laminin beta 1 antibody [EPR3189(2)] (ab108536) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : LAMB1 knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 198 kDa
    Observed band size: 200 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab108536).

    Lanes 1- 2: Merged signal (red and green). Green - ab108536 observed at 200 kDa. Red - Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) observed at 50 kDa.

     ab108536 was shown to react with Laminin beta 1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265414 (knockout cell lysate ab257499) was used. Wild-type HeLa and LAMB1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab108536 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Laminin beta 1 antibody [EPR3189(2)] - BSA and Azide free (ab247686)
    Western blot - Anti-Laminin beta 1 antibody [EPR3189(2)] - BSA and Azide free (ab247686)
    All lanes : Anti-Laminin beta 1 antibody [EPR3189(2)] (ab108536) at 1/1000 dilution

    Lane 1 : JAR cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : SW480 cell lysate
    Lane 4 : 293T cell lysate
    Lane 5 : C6 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 198 kDa



    This data was developed using ab108536, the same antibody clone in a different buffer formulation.

     

  • Anti-Laminin beta 1 antibody [EPR3189(2)] - BSA and Azide free (ab247686)
    Anti-Laminin beta 1 antibody [EPR3189(2)] - BSA and Azide free (ab247686)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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