Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Fibronectin antibody [EPR19241-46] (ab199056)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19241-46] to Fibronectin
Suitable for: WB, ICC/IF, IP, Flow Cyt, IHC-P
Reacts with: Mouse, Rat

Product name

Anti-Fibronectin antibody [EPR19241-46]
See all Fibronectin primary antibodies
Description

Rabbit monoclonal [EPR19241-46] to Fibronectin
Host species

Rabbit
Specificity

Western blot and IHC tests of human samples were negative.

Tested Applications & Species

Application	Species
Flow Cyt	Mouse
ICC/IF	Mouse
IHC-P	Mouse Rat
IP	Mouse
WB	Mouse Rat

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse and rat liver lysates; Mouse and rat plasma; Hepa1-6 whole cell lysate. IHC-P: Mouse and rat stomach tissues. ICC/IF: Hepa1-6 cells. Flow Cyt: Hepa1-6 cells. IP: Mouse plasma.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19241-46
Isotype

IgG
Research areas

Western blot - Anti-Fibronectin antibody [EPR19241-46] (ab199056)

All lanes : Anti-Fibronectin antibody [EPR19241-46] (ab199056) at 1/1000 dilution

Lane 1 : Mouse liver lysate
Lane 2 : Rat liver lysate
Lane 3 : Mouse plasma
Lane 4 : Rat plasma
Lane 5 : Hepa1-6 (mouse hepatoma epithelial cell line) whole cell lysate

Lysates/proteins at 10 µl/ml buffer per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Developed using the ECL technique.

Predicted band size: 273 kDa
Observed band size: 220-273 kDa
why is the actual band size different from the predicted?

Exposure time : Lanes 1 and 2:4 seconds; Lanes 3,4 and 5:2 seconds.

Blocking/Dilution buffer: 5% NFDM/TBST.

The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID: 6994838, PMID 7516997, PMID: 21923916).
Immunocytochemistry/ Immunofluorescence - Anti-Fibronectin antibody [EPR19241-46] (ab199056)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hepa 1-6 (mouse hepatoma epithelial cell line) cells labeling Fibronectin with ab199056 at 1/250 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and membranous staining on Hepa1-6 cells.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR19241-46] (ab199056)

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling Fibronectin with ab199056 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on the extracellular matrix of mouse stomach (PMID: 650151).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Fibronectin antibody [EPR19241-46] (ab199056)

Immunohistochemical analysis of paraffin-embedded rat stomach tissue labeling Fironectin with ab199056 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on the extracellular matrix of rat stomach (PMID: 650151).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cytometry - Anti-Fibronectin antibody [EPR19241-46] (ab199056)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized Hepa1-6 (mouse hepatoma epithelial cell line) cell line labeling Fibronectin with ab199056 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-Fibronectin antibody [EPR19241-46] (ab199056)

Fibronectin was immunoprecipitated from 0.35 mg of mouse plasma with ab199056 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab199056 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: Mouse plasma 10 µg (Input).

Lane 2: ab199056 IP in mouse plasma.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199056 in mouse plasma.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 1 second.
Anti-Fibronectin antibody [EPR19241-46] (ab199056)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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