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Signal Transduction Metabolism Energy Metabolism

Anti-DCXR antibody [4G4AF5] (ab110283)

Anti-DCXR antibody [4G4AF5] (ab110283)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [4G4AF5] to DCXR
  • Suitable for: WB, ICC/IF, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-DCXR antibody [4G4AF5]
    See all DCXR primary antibodies
  • Description

    Mouse monoclonal [4G4AF5] to DCXR
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue, cells or virus. This information is considered to be commercially sensitive.

  • Positive control

    • Isolated human liver mitochondria; Human HDFn cells; HL-60 cells.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Product was previously marketed under the MitoSciences sub-brand.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.5
    Preservative: 0.02% Sodium azide
    Constituent: HEPES buffered saline
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by affinity purification. Purity near homogeneity as judged by SDS-PAGE. Purity >95% by SDS-PAGE.
  • Clonality

    Monoclonal
  • Clone number

    4G4AF5
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Types of disease
    • Cancer

Images

  • Western blot - Anti-DCXR antibody [4G4AF5] (ab110283)
    Western blot - Anti-DCXR antibody [4G4AF5] (ab110283)
    Anti-DCXR antibody [4G4AF5] (ab110283) at 1 µg/ml + Human liver mitochondria at 20 µg

    Secondary
    Goat anti-mouse conjugated to AP

    Predicted band size: 26 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-DCXR antibody [4G4AF5] (ab110283)
    Immunocytochemistry/ Immunofluorescence - Anti-DCXR antibody [4G4AF5] (ab110283)
    Immunocytochemistry image of ab110283 stained Human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with ab110283 at 4 µg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (green) AlexaFluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in the cell membrane.
  • Flow Cytometry - Anti-DCXR antibody [4G4AF5] (ab110283)
    Flow Cytometry - Anti-DCXR antibody [4G4AF5] (ab110283)
    HL-60 cells were stained with 1 µg/mL ab110283 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-DCXR antibody [4G4AF5] (ab110283)

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    Anti-DCXR antibody (ab191369)

    Applications: IHC-P, WB

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    Anti-DCXR antibody (ab154290)

    Applications: IHC-P, WB

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