Anti-DCXR antibody [4G4AF5] (ab110283)
Key features and details
- Mouse monoclonal [4G4AF5] to DCXR
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-DCXR antibody [4G4AF5]
See all DCXR primary antibodies -
Description
Mouse monoclonal [4G4AF5] to DCXR -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus. This information is considered to be commercially sensitive.
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Positive control
- Isolated human liver mitochondria; Human HDFn cells; HL-60 cells.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by affinity purification. Purity near homogeneity as judged by SDS-PAGE. Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
4G4AF5 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Anti-DCXR antibody [4G4AF5] (ab110283) at 1 µg/ml + Human liver mitochondria at 20 µg
Secondary
Goat anti-mouse conjugated to AP
Predicted band size: 26 kDa
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Immunocytochemistry image of ab110283 stained Human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15min). The cells were incubated with ab110283 at 4 µg/ml for 2h at room temperature or over night at 4°C. The secondary antibody was (green) AlexaFluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in the cell membrane.
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HL-60 cells were stained with 1 µg/mL ab110283 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.