Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MIOX antibody [EPR17172] (ab199729)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17172] to MIOX
  • Suitable for: WB, IP
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-MIOX antibody [EPR17172]
    See all MIOX primary antibodies

  • Description

    Rabbit monoclonal [EPR17172] to MIOX

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal kidney lysate; Mouse kidney lysate. ICC/IF: HEK293 cells. IP: Mouse kidney whole cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-MIOX antibody [EPR17172] (ab199729)
    Western blot - Anti-MIOX antibody [EPR17172] (ab199729)
    All lanes : Anti-MIOX antibody [EPR17172] (ab199729) at 1/5000 dilution

    Lane 1 : Human fetal kidney tissue lysate
    Lane 2 : Human fetal heart tissue lysate
    Lane 3 : Human fetal spleen tissue lysate
    Lane 4 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 33 kDa
    Observed band size: 33 kDa


    Exposure time: 10 seconds


    Human fetal heart, Human fetal spleen and HeLa don’t express MIOX.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-MIOX antibody [EPR17172] (ab199729)
    Western blot - Anti-MIOX antibody [EPR17172] (ab199729)
    All lanes : Anti-MIOX antibody [EPR17172] (ab199729) at 1/5000 dilution

    Lane 1 : Mouse kidney tissue lysate
    Lane 2 : Mouse brain tissue lysate
    Lane 3 : Mouse heart tissue lysate
    Lane 4 : Mouse spleen tissue lysate
    Lane 5 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 6 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 33 kDa
    Observed band size: 33 kDa


    Exposure time: 10 seconds


    Mouse heart , mouse brain, mouse spleen, RAW264.7 and NIH/3T3 don’t express MIOX.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-MIOX antibody [EPR17172] (ab199729)
    Immunoprecipitation - Anti-MIOX antibody [EPR17172] (ab199729)

    MIOX was immunoprecipitated from 1mg of Mouse kidney whole cell lysate with ab199729 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab199729 at 1/2000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: Mouse kidney whole cell lysate 10 µg (Input). Lane 2: ab199729 IP in Mouse kidney whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199729 in Mouse kidney whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  • Anti-MIOX antibody [EPR17172] (ab199729)
    Anti-MIOX antibody [EPR17172] (ab199729)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-MIOX antibody [EPR17172] (ab199729)

© 2021 Astana Biomed Group LLP. All rights reserved.