Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CBL + CBLB antibody [YE315] (ab32425)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [YE315] to CBL + CBLB
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-CBL + CBLB antibody [YE315]

  • Description

    Rabbit monoclonal [YE315] to CBL + CBLB

  • Host species

    Rabbit

  • Specificity

    The immunogen sequence is 100% identical for both CBL and CBLB, therefore there is likely to be cross-reactivity.

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human CBL aa 450-550. The exact sequence is proprietary.
    Database link: P22681

  • Positive control

    • WB: HuT-78, HeLa and Jurkat cell lysate. IHC-P: Human uterus adenocarcinoma and colon tissues. ICC/IF: Jurkat cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Images

  • Western blot - Anti-CBL + CBLB antibody [YE315] (ab32425)
    Western blot - Anti-CBL + CBLB antibody [YE315] (ab32425)
    Anti-CBL + CBLB antibody [YE315] (ab32425) at 1/20000 dilution (purified) + HeLa cell lysate at 10 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 99 kDa
    Observed band size: 120 kDa
    why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBL + CBLB antibody [YE315] (ab32425)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBL + CBLB antibody [YE315] (ab32425)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human uterus adenocarcinoma tissue labelling CBL + CBLB with unpurified ab32425 at a dilution of 1/50.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-CBL + CBLB antibody [YE315] (ab32425)
    Immunocytochemistry/ Immunofluorescence - Anti-CBL + CBLB antibody [YE315] (ab32425)

    Immunocytochemistry/Immunofluorescence analysis of Jurkat cells labelling CBL + CBLB with purified ab32425 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.

    Control 1: primary antibody (1/250) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBL + CBLB antibody [YE315] (ab32425)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBL + CBLB antibody [YE315] (ab32425)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling CBL + CBLB with purified ab32425 at 1/50. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Western blot - Anti-CBL + CBLB antibody [YE315] (ab32425)
    Western blot - Anti-CBL + CBLB antibody [YE315] (ab32425)
    Anti-CBL + CBLB antibody [YE315] (ab32425) at 1/5000 dilution (purified) + HuT-78 cell lysate at 10 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 99 kDa
    Observed band size: 120 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-CBL + CBLB antibody [YE315] (ab32425)
    Western blot - Anti-CBL + CBLB antibody [YE315] (ab32425)
    Anti-CBL + CBLB antibody [YE315] (ab32425) at 1/500 dilution (unpurified) + Jurkat cell lysate

    Predicted band size: 99 kDa
    Observed band size: 120 kDa why is the actual band size different from the predicted?

  • Anti-CBL + CBLB antibody [YE315] (ab32425)
    Anti-CBL + CBLB antibody [YE315] (ab32425)

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