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Human HSPB8 (Hsp22) knockout HeLa cell pellet (ab278954)

Overview

  • Product name

    Human HSPB8 (Hsp22) knockout HeLa cell pellet
    See all Hsp22/HSPB8 kits

  • Product overview

    Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.

    Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation.

  • Parental Cell Line

    HeLa

  • Organism

    Human

  • Mutation description

    Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 2 bp deletion in exon1.

  • Passage number

  • Knockout validation

    Sanger Sequencing

  • Notes

    Pellet size: 5 million cells/vial.

    This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

Properties

Target

  • Function

    Displays temperature-dependent chaperone activity.

  • Tissue specificity

    Predominantly expressed in skeletal muscle and heart.

  • Involvement in disease

    Neuronopathy, distal hereditary motor, 2A
    Charcot-Marie-Tooth disease 2L

  • Sequence similarities

    Belongs to the small heat shock protein (HSP20) family.

  • Cellular localization

    Cytoplasm. Nucleus. Translocates to nuclear foci during heat shock.
  • Information by UniProt

  • Alternative names

    • Alpha crystallin C chain
    • Alpha-crystallin C chain
    • Charcot Marie Tooth disease axonal type 2L
    • Charcot Marie Tooth disease spinal
    • CMT2L
    • CRYAC
    • DHMN 2
    • DHMN2
    • E2 induced gene 1 protein
    • E2-induced gene 1 protein
    • E2IG1
    • H11
    • Heat shock 22kDa protein 8
    • Heat shock 27kDa protein 8
    • Heat shock protein 22
    • Heat shock protein beta 8
    • Heat shock protein beta-8
    • Hereditary motor neuropathy distal
    • HMN 2
    • HMN2
    • HMN2A
    • HSB8
    • HSPB 8
    • HspB8
    • HSPB8_HUMAN
    • OTTHUMP00000239768
    • Protein kinase H11
    • Small stress protein like protein HSP22
    • Small stress protein-like protein HSP22
    • Spinal muscular atrophy distal adult autosomal dominant
    see all

Properties

Images

  • Sanger Sequencing - Human HSPB8 (Hsp22) knockout HeLa cell pellet (ab278954)
    Sanger Sequencing - Human HSPB8 (Hsp22) knockout HeLa cell pellet (ab278954)

    Allele-1: 2 bp deletion in exon1

     

  • Sanger Sequencing - Human HSPB8 (Hsp22) knockout HeLa cell pellet (ab278954)
    Sanger Sequencing - Human HSPB8 (Hsp22) knockout HeLa cell pellet (ab278954)

    Allele-2: 1 bp deletion in exon1

     

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