Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Midkine antibody [EP1143Y] (ab52637)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1143Y] to Midkine
  • Suitable for: WB, IP, Flow Cyt, IHC-P, ICC/IF
  • Reacts with: Human, Recombinant fragment

Overview

  • Product name

    Anti-Midkine antibody [EP1143Y]
    See all Midkine primary antibodies

  • Description

    Rabbit monoclonal [EP1143Y] to Midkine

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human Midkine aa 100 to the C-terminus (C terminal).

  • Positive control

    • Human liver carcinoma; Human pancreatic carcinoma; HeLa cells; SH-SY5Y (Human neuroblastoma cell line from bone marrow).

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EP1143Y

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Midkine antibody [EP1143Y] (ab52637)
    Western blot - Anti-Midkine antibody [EP1143Y] (ab52637)
    Anti-Midkine antibody [EP1143Y] (ab52637) at 1/500 dilution (unpurified) + Recombinant protein at 0.001 µg

    Secondary
    Goat anti-rabbit HRP labeled at 1/2000 dilution

    Predicted band size: 16 kDa
    Observed band size: 16 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Midkine antibody [EP1143Y] (ab52637)
    Immunocytochemistry/ Immunofluorescence - Anti-Midkine antibody [EP1143Y] (ab52637)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Midkine with purified ab52637 at 1:100 dilution (10.5μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunoprecipitation - Anti-Midkine antibody [EP1143Y] (ab52637)
    Immunoprecipitation - Anti-Midkine antibody [EP1143Y] (ab52637)

    ab52637 (purified) at 1:20 dilution (2ug) immunoprecipitating Midkine in SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate.

    Lane 1 (input): SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate 10ug

    Lane 2 (+): ab52637 & SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52637 in SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:5000 dilution.

    Blocking and diluting buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Midkine antibody [EP1143Y] (ab52637)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Midkine antibody [EP1143Y] (ab52637)

    Human liver carcinoma stained with unpurified ab52637 at 1/100 - 1/250 dilution

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-Midkine antibody [EP1143Y] (ab52637)
    Western blot - Anti-Midkine antibody [EP1143Y] (ab52637)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human pancreatic carcinoma tissue sections labeling Midkine with purified ab52637 at 1:50 dilution (2.1 μg/ml). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.

  • Western blot - Anti-Midkine antibody [EP1143Y] (ab52637)
    Western blot - Anti-Midkine antibody [EP1143Y] (ab52637)
    Anti-Midkine antibody [EP1143Y] (ab52637) at 1/1000 dilution (purified) + SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 16 kDa
    Observed band size: 16 kDa



    Blocking and diluting: 5% NFDM/TBST

  • Western blot - Anti-Midkine antibody [EP1143Y] (ab52637)
    Western blot - Anti-Midkine antibody [EP1143Y] (ab52637)
    Anti-Midkine antibody [EP1143Y] (ab52637) at 1/10000 dilution (purified) + Recombinant Human Midkine protein (ab49848) at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 16 kDa
    Observed band size: 16 kDa



    Blocking and diluting buffer: 5% NFDM/TBST
  • Flow Cytometry - Anti-Midkine antibody [EP1143Y] (ab52637)
    Flow Cytometry - Anti-Midkine antibody [EP1143Y] (ab52637)

    Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Midkine with purified ab52637 at 1/250 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Immunocytochemistry/ Immunofluorescence - Anti-Midkine antibody [EP1143Y] (ab52637)
    Immunocytochemistry/ Immunofluorescence - Anti-Midkine antibody [EP1143Y] (ab52637)

    HeLa cells stained with unpurified ab52637 at 1/250 - 1/500 dilution

  • Anti-Midkine antibody [EP1143Y] (ab52637)
    Anti-Midkine antibody [EP1143Y] (ab52637)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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